P6065-1mg
Recombinant Murine sTNF RII/TNFRSF1B
1mg
15611.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
21938
P25119
E. coli
236aa
25.3kDa
－
Name
Recombinant Murine sTNF RII/TNFRSF1B (Recombinant Murine soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B; rMusTNF RII/TNFRSF1B)；Recombinant Mouse sTNF RII/TNFRSF1B ; 重组小鼠可溶性肿瘤坏死因子受体II型/TNFRSF1B
Synonyms
CD120b; TNFRSF1B; CD120b; CD120b antigen; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNF-RII; TNF-R-II; TNFR-II; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 2μg/ml, corresponding to a specific activity of >500IU/mg in the presence of 0.1ng/ml of rMuTNF-α.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rMusTNF RII/TNFRSF1B as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
TNFRSF1B also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is encoded by the TNFRSF1B gene in humans. It is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. TNFRSF1B has been shown to interact with TRAF2 and TTRAP. Recombinant murine TNFRSF1B is a 25.3kDa protein with 236 amino acid residues.
Amino Acid Sequence
VPAQVVLTPY KPEPGYECQI SQEYYDRKAQ MCCAKCPPGQ YVKHFCNKTS DTVCADCEAS MYTQVWNQFR TCLSCSSSCT TDQVEIRACT KQQNRVCACE AGRYCALKTH SGSCRQCMRL SKCGPGFGVA SSRAPNGNVL CKACAPGTFS DTTSSTDVCR PHRICSILAI PGNASTDAVC APESPTLSAI PRTLYVSQPE PTRSQPLDQE PGPSQTPSIL TSLGSTPIIE QSTKGG
包装清单:
P6065-5μg
Recombinant Murine sTNF RII/TNFRSF1B
5μg
P6065-20μg
Recombinant Murine sTNF RII/TNFRSF1B
20μg
P6065-100μg
Recombinant Murine sTNF RII/TNFRSF1B
100μg
P6065-1mg
Recombinant Murine sTNF RII/TNFRSF1B
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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