P6992-10μg
Recombinant Murine Prolactin
10μg
464.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
19109
Q3TT66
E. coli
197aa
22.4kDa
－
Name
Recombinant Murine Prolactin (Recombinant Murine Prolactin; rMuProlactin)； Recombinant Mouse Prolactin ; 重组小鼠催乳素
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rMuProlactin as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Prolactin, also named PRL and lactotrope is a peptide hormone, encoded by the PRL gene. The structure of prolactin is similar to that of growth hormone and placental lactogen. There are mainly three different forms of prolactin in regard to size: little prolactin, big prolactin and big big prolactin. Little prolactin is the predominant form. Although often associated with human milk production, prolactin plays a wide range of other roles in both humans and other verterbrates. Recombinant murine prolactin is a 22.4kDa globular protein containing 197 amino acid residues and shares 77%~90%a.a. sequence identity with human and rat prolactin.
Amino Acid Sequence
LPICSAGDCQ TSLRELFDRV VILSHYIHTL YTDMFIEFDK QYVQDREFMV KVINDCPTSS LATPEDKEQA LKVPPEVLLN LILSLVQSSS DPLFQLITGV GGIQEAPEYI LSRAKEIEEQ NKQLLEGVEK IISQAYPEAK GNGIYFVWSQ LPSLQGVDEE SKILSLRNTI RCLRRDSHKV DNFLKVLRCQ IAHQNNC
包装清单:
P6992-10μg
Recombinant Murine Prolactin
10μg
P6992-50μg
Recombinant Murine Prolactin
50μg
P6992-100μg
Recombinant Murine Prolactin
100μg
P6992-1mg
Recombinant Murine Prolactin
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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