P6110-20μg
Recombinant Murine NOV
20μg
1152.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
18133
Q64299
E. coli
333aa
36.4kDa
－
Name
Recombinant Murine NOV (Recombinant Murine Nephroblastomaoverexpressed Gene Protein Homolog; rMuNOV)；Recombinant Mouse NOV ; 重组小鼠成肾肾母细胞瘤过度表达基因同源蛋白
Synonyms
CCN3; IGFBP-9; CCN family member 3; CCN3IGF-binding protein 9; IBP-9; IGFBP-9; IGFBP9novH; Insulin-like growth factor-binding protein 9; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOVH; NovH; protein NOV homolog
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0μg/ml, corresponding to a specific activity of >1000IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH8.6, 150mM NaCl.
Endotoxin
Less than 0.1EU/μg of rMuNOV as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
NOV, also named CCN3, IGFBP-9 and NovH, is belonging to the CCN (CTGF/CYR61/NOV) family and it is encoded by the NOV gene. It is expressed in certain tumors, including Wilm's tumor and most nephroblastomas. NOV interacts with several proteins and is involved in both internal and external cell signaling. NOV plays an important role in reducing tumorgenicity and proliferation of certain cancer cell lines. It also been reported to exert proangiogenic activities. Recombinant murine NOV is an 36.4kDa protein containing 333 amino acid residues and it shares 92% and 82%a.a. identity with rat and human NOV.
Amino Acid Sequence
QVSASLRCPS RCPPKCPSIS PTCAPGVRSV LDGCSCCPVC ARQRGESCSE MRPCDQSSGL YCDRSADPNN QTGICMVPEG DNCVFDGVIY RNGEKFEPNC QYFCTCRDGQ IGCLPRCQLD VLLPGPDCPA PRKVAVPGEC CEKWTCGSDE QGTQGTLGGL ALPAYRPEAT VGVEVSDSSI NCIEQTTEWS ACSKSCGMGV STRVTNRNRQ CEMVKQTRLC IVRPCEQEPE EVTDKKGKKC LRTKKSLKAI HLQFENCTSL YTYKPRFCGV CSDGRCCTPH NTKTIQVEFQ CLPGEIIKKP VMVIGTCTCY SNCPQNNEAF LQDLELKTSR GEI
包装清单:
P6110-5μg
Recombinant Murine NOV
5μg
P6110-20μg
Recombinant Murine NOV
20μg
P6110-100μg
Recombinant Murine NOV
100μg
P6110-1mg
Recombinant Murine NOV
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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