P6798-20μg
Recombinant Murine MIP-1γ/CCL9/CCL10
20μg
1152.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
20308
P51670
E. coli
101aa
11.6kDa
－
Name
Recombinant Murine MIP-1γ/CCL9/CCL10 (Recombinant Murine Macrophage Inflammatory Protein-1γ/CCL9/CCL10; rMuMIP-1γ/CCL9/CCL10)；Recombinant Mouse MIP-1γ/CCL9/CCL10 ; 重组小鼠巨噬细胞炎症蛋白1γ/CCL9/CCL10
Synonyms
CCF18; MIP-1 gamma
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51% sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75% amino acid identical to rat CCL9/10.
Amino Acid Sequence
QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q
包装清单:
P6798-5μg
Recombinant Murine MIP-1γ/CCL9/CCL10
5μg
P6798-20μg
Recombinant Murine MIP-1γ/CCL9/CCL10
20μg
P6798-100μg
Recombinant Murine MIP-1γ/CCL9/CCL10
100μg
P6798-1mg
Recombinant Murine MIP-1γ/CCL9/CCL10
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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