P5957-1mg
Recombinant Murine IL-21
1mg
26773.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
60505
Q9ES17
E. coli
129aa
15kDa
－
Name
Recombinant Murine IL-21 (Recombinant Murine Interleukin-21; rMuIL-21)；Recombinant Mouse IL-21 ; 重组小鼠白细胞介素21
Synonyms
IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25ng/ml, corresponding to a specific activity of >4.0×104IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rMuIL-21 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
IL-21 is encoded by IL-21 gene located on Chr. 4 in mouse. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. The murine IL-21 precursor contains a predicted 17 amino acid (a.a.) signal sequence and a 129a.a. mature chain. Mature murine IL-21 shares 66%, 59%, 58% and 88%a.a. sequence identity with mature canine, human, rabbit and rat IL-21, respectively.
Amino Acid Sequence
HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS
包装清单:
P5957-2μg
Recombinant Murine IL-21
2μg
P5957-10μg
Recombinant Murine IL-21
10μg
P5957-100μg
Recombinant Murine IL-21
100μg
P5957-1mg
Recombinant Murine IL-21
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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