P6192-2μg
Recombinant Murine GDF-7/BMP-12
2μg
618.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
238057
P43029
E. coli
146aa
29.8kDa
－
Name
Recombinant Murine GDF-7/BMP-12 (Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12; rMuGDF-7/BMP-12)；Recombinant Mouse GDF-7/BMP-12 ; 重组小鼠生长分化因子7/人骨形态发生蛋白12
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5μg/ml, corresponding to a specific activity of >2000IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
Endotoxin
Less than 0.1EU/μg of rMuGDF-7/BMP-12 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
Amino Acid Sequence
TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
包装清单:
P6192-2μg
Recombinant Murine GDF-7/BMP-12
2μg
P6192-10μg
Recombinant Murine GDF-7/BMP-12
10μg
P6192-100μg
Recombinant Murine GDF-7/BMP-12
100μg
P6192-1mg
Recombinant Murine GDF-7/BMP-12
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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