P6101-1mg
Recombinant Murine FGF-21
1mg
15611.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
56636
Q9JJN1
E. coli
182aa
19.9kDa
-
Name
Recombinant Murine FGF-21 (Recombinant Murine Fibrobalst Growth Factor-21; rMuFGF-21);Recombinant Mouse FGF-21 ; 重组小鼠成纤维细胞生长因子21
Synonyms
FGF-21; fibroblast growth factor 21
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/ml, corresponding to a specific activity of >2.0×103IU/mg in the presence of 5μg/ml of rMuKlotho-β and 10μg/ml of heparin.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 3X PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rMuFGF-21 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Recombinant murine FGF-21 contains 182 amino acids residues and show limited binding to heparin. In addition, Murine FGF-21 respectively shows 81% and 92%a.a. identity to human and rat FGF-21, and it show activity on human and rat cells.
Amino Acid Sequence
AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS
包装清单:
P6101-5μg
Recombinant Murine FGF-21
5μg
P6101-25μg
Recombinant Murine FGF-21
25μg
P6101-100μg
Recombinant Murine FGF-21
100μg
P6101-1mg
Recombinant Murine FGF-21
1mg
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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