P7338-1mg
Recombinant Murine DHH C23II
1mg
15444.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
13363
Q61488
E. coli
177aa
20kDa
－
Name
Recombinant Murine DHH C23II (Recombinant Murine Desert Hedgehog Cys23IleIle; rMuDHH C23II)； Recombinant Mouse DHH C23II ; 重组小鼠沙漠刺猬因子C23II
Synonyms
DHH; desert hedgehog; desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; HHG-3MGC35145
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells is 5-20μg/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 1mM DTT, 0.05% Tween-80.
Endotoxin
Less than 0.1EU/μg of rMuDHH C23II as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Desert hedgehog protein also known as HHG-3 is a member of the hedgehog family which is encoded by the DHH gene. It is expressed in adult testes but not expressed in limb buds. DHH may be as a precursor that is auto-catalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. Besides, DHH may have a function as a spermatocyte survival factor in the testes. Within the N-terminal peptide, murine DHH shares 97% and 100% amino acid (a.a.) sequence identity with human and rat DHH, respectively.
Amino Acid Sequence
IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG
包装清单:
P7338-5μg
Recombinant Murine DHH C23II
5μg
P7338-25μg
Recombinant Murine DHH C23II
25μg
P7338-100μg
Recombinant Murine DHH C23II
100μg
P7338-1mg
Recombinant Murine DHH C23II
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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