| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P5563-10μg |
Recombinant Human VEGF165 |
10μg |
1152.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
7422
|
P15692-4
|
Yeast
|
166aa
|
20~31kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Human VEGF165 (Recombinant Human Vascular Endothelial Growth Factor 165; rHuVEGF165)；重组人血管内皮生长因子165
|
|
Synonyms
|
Vascular Endothelial Growth Factor Isoform 165; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF
|
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 0.02% Tween-20.
|
|
Endotoxin
|
Less than 0.1EU/μg of rHuVEGF165 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (a.a.) in length. VEGF production can be induced in cells that are not receiving enough oxygen. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Recombinant human VEGF165 contains 166 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 88%a.a. with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively.
|
|
Amino Acid Sequence
|
MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P5563-2μg
|
Recombinant Human VEGF165
|
2μg
|
|
P5563-10μg
|
Recombinant Human VEGF165
|
10μg
|
|
P5563-100μg
|
Recombinant Human VEGF165
|
100μg
|
|
P5563-1mg
|
Recombinant Human VEGF165
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。