| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P7104-100μg |
Recombinant Human UBE2D3, His-tag |
100μg |
3214.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
7323
|
P61077
|
E. coli
|
155aa
|
17.7kDa
|
His
|
|
About this Antibody
|
|
Name
|
Recombinant Human UBE2D3, His-tag (Recombinant Human Ubiquitin Conjugating Enzyme E2 D3, His-tag; rHuUBE2D3, His-tag)；重组人泛素结合酶E2D3，His标签
|
|
Synonyms
|
UBC5C; UBE2D3; E2(17)KB 3; E2(17)KB3; EC 6.3.2.19; MGC43926; MGC5416; UBC4/5; UBC5C; UbcH5C; UBCH5C; Ubiquitin carrier protein D3; ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3
|
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Data is not available.
|
|
Physical Appearance
|
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
|
|
Formulation
|
A 0.2μm filtered concentrated solution in 50mM HEPES, pH6.5, with 125mM NaCl, 10% Glycerol, 5% Trehalose, 1mM DTT.
|
|
Endotoxin
|
Less than 1EU/μg of rHuUBE2D3/UBC5C, His-tag as determined by LAL method.
|
|
Reconstitution
|
N/A
|
|
Category
|
Ubiquitins
|
|
Background
|
Ubiquitin-conjugating enzyme E2 D3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2D3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2D3 is a human homolog of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. It mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins and has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively.
|
|
Amino Acid Sequence
|
MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P7104-10μg
|
Recombinant Human UBE2D3, His-tag
|
10μg
|
|
P7104-50μg
|
Recombinant Human UBE2D3, His-tag
|
50μg
|
|
P7104-100μg
|
Recombinant Human UBE2D3, His-tag
|
100μg
|
|
P7104-1mg
|
Recombinant Human UBE2D3, His-tag
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。