产品编号 |
产品名称 |
产品包装 |
产品价格 |
P7176-10μg |
Recombinant Human Rb137, His-tag |
10μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
5925
|
P06400
|
E. coli
|
146aa
|
16.5kDa
|
His
|
About this Antibody
|
Name
|
Recombinant Human Rb137, His-tag (Recombinant Human Retinoblastoma-Associated
Protein Fragment, 137a.a. with His-tag; rHuRb137, His-tag);重组人视网膜母细胞瘤相关蛋白片段137a.a.,His标签
|
Synonyms
|
RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
N/A
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.
|
Endotoxin
|
Less than 1EU/μg of rHuRb137, His-tag as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Others
|
Background
|
Rb encoded by the RB1 gene in humans, is expressed by retina and belongs to the etinoblastoma-associated protein family. The hole protein consists of 928a.a. and the rHuRb fragment occupies sequence of 792-929a.a.. Rb is a key regulator of entry into cell division that acts as a tumor suppressor. It has many functions, for example, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C, and acts as a transcription repressor of E2F1 target genes and so on. The rHuRb is the region that rich of modified residue like phosphothreonine and N6-acetyllysine.
|
Amino Acid Sequence
|
MASFPSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGSNP PKPLKKLRFD IEGSDEADGS KHLPGESKFQ QKLAEMTSTR TRMQKQKMND SMDTSNKEEK HHHHHH
|
包装清单:
产品编号
|
产品名称
|
包装
|
P7176-10μg
|
Recombinant Human Rb137, His-tag
|
10μg
|
P7176-50μg
|
Recombinant Human Rb137, His-tag
|
50μg
|
P7176-100μg
|
Recombinant Human Rb137, His-tag
|
100μg
|
P7176-1mg
|
Recombinant Human Rb137, His-tag
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。