| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P7266-20μg |
Recombinant Human MIA |
20μg |
1159.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
8190
|
Q16674
|
E. coli
|
107aa
|
12.1kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Human MIA (Recombinant Human Melanoma Inhibitor Activity Protein; rHuMIA)；重组人黑色素瘤活性抑制蛋白
|
|
Synonyms
|
CD-RAP; CD-RAP; melanoma inhibitory activity; Melanoma inhibitory activity protein; melanoma-derived growth regulatory protein
|
|
Purity
|
>98% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5μg/ml, corresponding to a specific activity of >200IU/mg.
|
|
Physical Appearance
|
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 5% Trehalose.
|
|
Endotoxin
|
Less than 0.1EU/μg of rHuMIA as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Others
|
|
Background
|
MIA is an autocrine growth regulatory protein, secreted from chondrocytes and malignant melanoma cells, that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis, as well as a potential marker for rheumatoid arthritis. The MIA is a member of the MIA/OTOR family, which also includes MIA, OTOR, and TANGO, and they share a Src homology-3 (SH3)-like domain. Recombinant Human MIA is a 12.1kDa globular protein containing 107 amino acid residues, including two intramolecular disulfide bonds.
|
|
Amino Acid Sequence
|
GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P7266-5μg
|
Recombinant Human MIA
|
5μg
|
|
P7266-20μg
|
Recombinant Human MIA
|
20μg
|
|
P7266-100μg
|
Recombinant Human MIA
|
100μg
|
|
P7266-1mg
|
Recombinant Human MIA
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。