| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P5205-1mg |
Recombinant Human IL-17B |
1mg |
15444.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
27190
|
Q9UHF5
|
E. coli
|
161aa
|
36.5kDa
|
－
|
|
About this protein
|
|
Name
|
Recombinant Human IL-17B (Recombinant Human Interleukin-17B; rHuIL-17B)；重组人白细胞介素17B
|
|
Synonyms
|
Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20
|
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion of human HepG2 cells is less than 1.0μg/ml, corresponding to a specific activity of >1000IU/mg.
|
|
Physical Appearance
|
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 0.1% Tween-20 and 3% Trehalose.
|
|
Endotoxin
|
Less than 0.1EU/μg of rHuIL-17B as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
IL-17B, also named IL20, NIRF and ZCYTO7, is belonging to the IL-17 family and it stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. IL-17B mRNA is expressed in adult pancreas, small intestine, and stomach. The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.
|
|
Amino Acid Sequence
|
MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P5205-5μg
|
Recombinant Human IL-17B
|
5μg
|
|
P5205-25μg
|
Recombinant Human IL-17B
|
25μg
|
|
P5205-100μg
|
Recombinant Human IL-17B
|
100μg
|
|
P5205-1mg
|
Recombinant Human IL-17B
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。