| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P7163-100μg |
Recombinant Human HMGB1, His-tag |
100μg |
3214.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
3146
|
P09429
|
E. coli
|
223aa
|
26kDa
|
His
|
|
About this Antibody
|
|
Name
|
Recombinant Human HMGB1, His-tag (Recombinant Human High-Mobility Group Box 1, His-tag; rHuHMGB1, His-tag)；重组人高移动性型盒2，His标签
|
|
Synonyms
|
HMG-1; Amphoterin; high mobility group box 1; High mobility group protein 1; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG1DKFZp686A04236; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
|
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Data not available.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
|
Endotoxin
|
Less than 1EU/μg of rHuHMGB1, His-tag as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Others
|
|
Background
|
Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30kDa, 215 amino acid (a.a.) single chain polypeptide containing three domains: two N-terminal globular, 70a.a. positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30a.a. C-terminal region that contains only Asp and Glu.4, 5 Residues 27-43 and 178-184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
|
|
Amino Acid Sequence
|
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P7163-10μg
|
Recombinant Human HMGB1, His-tag
|
10μg
|
|
P7163-50μg
|
Recombinant Human HMGB1, His-tag
|
50μg
|
|
P7163-100μg
|
Recombinant Human HMGB1, His-tag
|
100μg
|
|
P7163-1mg
|
Recombinant Human HMGB1, His-tag
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。