| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P6474-100μg |
Recombinant Equine IL-1RA |
100μg |
1491.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Equine
|
100034236
|
O18999
|
E. coli
|
152aa
|
17.4kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Equine IL-1RA (Recombinant Equine Interleukin-1 Receptor Antagonist; rEqIL-1RA)；重组马白细胞介素1受体拮抗剂
|
|
Synonyms
|
IL-1F3; IL1RN; DIRA; ICIL-1RA; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4
|
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 3.0μg/ml, corresponding to a specific activity of >333IU/mg in the presence of 50pg/ml rHuIL-1α.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
|
Endotoxin
|
Less than 1EU/μg of rEqIL-1RA as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. It is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-1RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The equus caballus IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26% amino acid homology to IL-1β and 19% homology to IL-1α. It also shares 78%~80%a.a. sequence identity with murine, rat, porcine IL-1RA.
|
|
Amino Acid Sequence
|
HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P6474-5μg
|
Recombinant Equine IL-1RA
|
5μg
|
|
P6474-20μg
|
Recombinant Equine IL-1RA
|
20μg
|
|
P6474-100μg
|
Recombinant Equine IL-1RA
|
100μg
|
|
P6474-1mg
|
Recombinant Equine IL-1RA
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。