| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| AF1762 |
Phospho-JNK1/JNK2/JNK3 (Thr183/Thr183/Thr221) Rabbit Monoclonal Antibody |
50μl |
618.00元 |
|
来源
|
用途
|
交叉反应性
|
分子量
|
|
Rabbit
|
WB, IP, IF, IHC, ICC, FC
|
H, M, R
|
54/46 kDa
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:2,000
|
1:10-1:50
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
1:50-1:100
|
-
|
-
|
抗体详细信息如下:
|
About this Antibody
|
|
Name
|
Phospho-JNK1/JNK2/JNK3 (Thr183/Thr183/Thr221) Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
Affinity purification
|
|
About the Immunogen
|
|
Immunogen
|
Recombinant protein
|
|
Gene ID
|
5599/5601/5602(Human); 26414/26419/26420(Mouse); 116554/50658/25272(Rat)
|
|
SwissProt
|
P45983/P45984/P53779(Human); Q61831/Q91Y86/Q9WTU6(Mouse); P49185/P49186/P49187(Rat)
|
|
Synonyms
|
C Jun kinase 2; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal kinase 3; c-Jun N-terminal kinase 1; JNK 46; JNK 55; JNK; MAP kinase 10; MAP kinase 9; MAP kinase 8;Mitogen activated protein kinase 10; Mitogen activated protein kinase 9; Mitogen activated protein kinase 8; PRKM9; PRKM8; PRKM10
|
|
Category
|
MAPK
|
|
Background
|
JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
AF1762
|
Phospho-JNK1/JNK2/JNK3 (Thr183/Thr183/Thr221) Rabbit Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀释液
|
50ml
|
|
-
|
说明书
|
1份
|
保存条件:
Phospho-JNK1/JNK2/JNK3 (Thr183/Thr183/Thr221) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。