Atg5 Antibody

品牌:CST，产品名称：Atg5 Antibody #2630
英文名称：Atg5 Antibody
Product name：Atg5 Antibody #2630
Source：Rabbit
Sensitivity：Endogenous
MW(kDa)：55
Reactivity：H,Mk
Applications：WB , IP ,
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of Atg5. Antibodies were purified by peptide affinity chromatography.
Background： Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Entrez-Gene Id

9474

Swiss-Prot Acc.

Q9H1Y0

Specificity / Sensitivity:

Atg5 Antibody detects endogenous levels of total Atg5. The observed band represents the Atg12-Atg5 conjugated form, but the antibody likely reacts with free Atg5 as well.

Species Reactivity:

Human, Monkey

Application	Dilutions
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.