5' | | | R ↓ | C | C | G | G | Y | | 3' | |
3' | | | Y | G | G | C | C ↑ | R | | 5' | |
Thermo Scientific Cfr10I (BsrFI) restriction enzyme recognizes R^CCGGY sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Bse118I, BsrFI, BssAIm). See
Reaction Conditions for Restriction Enzymes
for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a
FastDigest enzyme
for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
关键字: Cfr10I (BsrFI) (10 U/μL);分子生物学
北京索莱宝科技有限公司是一家集研发、生产、销售、服务于一体的高科技生物企业。技术推广;技术转让;技术咨询;技术服务;生物试剂技术开发;销售生物试剂盒、实验室专用化学试剂、实验室专用设备、办公用品、化工产品(不含危险化学品)、计算机软件及辅助设备;货物进出口、技术进出口、代理进出口;委托加工生产生物制品、生化制品、化工产品(不含危险化学品);生产生物试剂盒;批发第一类医疗器械、第二类医疗器械;批发第三类医疗器械。