细胞自噬是真核生物在保守进化中对细胞内物质进行周转的重要过程，该过程中一些损坏的蛋白或细胞器被双层膜结构的自噬小泡包裹后，送入溶酶体中进行降解并得以循环利用。动物细胞衰老反应过程中，往往发生分子伴侣介导的自噬过程，细胞自噬与细胞凋亡、细胞衰老一样，是十分重要的生物学现象，它参与生物的发育、生长等多种过程，细胞自噬的异常易导致癌细胞的出现。ProSci有很多种细胞自噬抗体，其中包括自噬损害调节器DRAM抗体和自噬相关蛋白APG7、PIST、LAMP-1、LAMP-2抗体。此外，ProSci还提供自噬检测试剂盒。ProSci的每种自噬抗体都可适用于ELISA，western blot和免疫组织化学（IHC）。
Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
Description
Western blot analysis of Beclin-1 in 293 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of beclin-1 in rat brain tissue with beclin-1 antibody at 2 μg/mL.
Additional Names
Beclin-1 Antibody: Beclin-1, Coiled-coil myosin-like Bcl-2-interacting protein
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid
Concentration: 1 mg/mL
Isotype
IgG
Clonality/Clone
This is a polyclonal Beclin-1 Antibody.
Conjugate
None
Purification
Beclin-1 Antibody is affinity chromatography purified via peptide column.
Host
Beclin-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Beclin-1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1.
Application
Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. Tested Application
E, WB, IHC
Buffer
Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3613P - Beclin-1 Peptide
Storage
Beclin-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Positive Control
- Cat. No. 1210 - 293 Cell Lysate
- Cat. No. 1403 - Mouse Brain Tissue Lysate]
Species Reactivity
H, M, R
Protein GI Number
16307457
Protein Accession Number
AAH10276 Reference
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
- Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91.]