应用:
透射电镜/扫描透射电镜、光学显微镜、蛋白杂交、纯化his标签蛋白时,识别含目的蛋白的部分等实验。
优点:
灵敏度高
探针小,分辨率高,1.8 nm Ni-NTA-纳米金比抗体探针小
高溶解度,稳定
无背景
永久染色
5 nm Ni-NTA-纳米金在电镜下可见,不需金增强/银增强
使用简单 ![Structure and STEM (39k)](http://www.bioconsumable.com/admin/webedit/UploadFile/201459153318939.jpg)
左:1.8nm Ni-NTA-纳米金与his标签蛋白相互作用右:用Ni-NTA-纳米金标记6xhis腺病毒Knob蛋白,未染色的扫描电镜照片
left: Structure of Ni-NTA-Nanogold® showing interaction with Interaction with a His-tagged protein; right: Knob protein from adenovirus cloned with 6x-His tag, labeled with Ni-NTA-Nanogold, column purified from excess gold, and viewed in the scanning transmission electron microscope (STEM) unstained (Full width approximately 245 nm).
![](http://www.bioconsumable.com/admin/webedit/UploadFile/201459153347173.jpg)
上:5nm Ni-NTA-纳米金的结构,显示金属螯合物与his标签蛋白结合
下:5nm Ni-NTA-纳米金的扫描电镜图,平均直径5.11±0.84nm。
Top: Structure of NTA-Ni(II)-5 nm Nanogold®, showing the binding of the incorporated metal chelate to a His-tagged protein; distance from the gold particle surface to the His tag is estimated to be 1.5 nm. Above: Transmission electron micrograph of 5 nm NTA Nanogold: average diameter 5.11±0.84nm.
品名 | 产品描述 | 货号 | 规格 |
1.8 nm Ni-NTA-Nanogold® | 比抗体小,更易渗透入组织,定位和检测细胞、组织或蛋白复合物中的his标签蛋白。 | 2080 | 10uM 30ml |
5 nm Ni-NTA-Nanogold® | 目标量达一定程度时,无需银增强/金增强可直接用电镜观察 | 2082 | 0.5uM 3ml |
相关产品:
GoldEnhance LM/Blot (GELM) | 金增强试剂,用于光镜样品 | 2112 | Initiator/Moderator/Activator/Buffer 各15 ml(共60 ml,足够做600张载玻片) |
GoldEnhance EM(GEEM) | 金增强试剂,用于电镜样品 | 2113 | Initiator/Moderator/Activator/Buffer 各2 ml(共8 ml,足够做200个网格) |
HQ Silver | 用于纳米金的质量**的增强试剂,均匀显影,极好的保持结构,特别适合电镜,光敏感 | 2012 | Initiator/Moderator/Activator 各15 ml(共45 ml) |
Li Silver | 纳米金的银增强,用于电镜、光镜、凝胶、杂交,光不敏感 | 2013 | Initiator/Enhancer 125 ml (共250 ml) |
References:
1. Kollman, J. M.; Zelter, A.; Muller, E. G.; Fox, B.; Rice, L. M.; Davis, T. N., and Agard, D. A.: The Structure of the gamma-Tubulin Small Complex: Implications of Its Architecture and Flexibility for Microtubule Nucleation. Mol. Biol. Cell, 19, 207-215 (2008).
2. Adami, A.; Garcia-Alvarez, B.; Arias-Palomo, E.; Barford, D., and Llorca, O.: Structure of TOR and its complex with KOG1. Mol. Cell., 27509-516 (2007).
3. Balasingham, S. V.; Collins, R. F.; Assalkhou, R.; Homberset, H.; Frye, S. A.; Derrick, J. P., and Tonjum, T.: Interactions between the Lipoprotein PilP and the Secretin PilQ in Neisseria meningitidis. J. Bacteriol., 189, 5716-5727 (2007).
4. Jiang, Z. G.; Simon, M. N.; Wall, J. S., and McKnight, C. J.: Structural analysis of reconstituted lipoproteins containing the N-terminal domain of apolipoprotein B. Biophys. J., 92, 4097-4108 (2007).
5. Pye, V. E, Beuron, F, Keetch, C. A, McKeown, C, Robinson, C. V, Meyer, H. H, Zhang, X, and Freemont, P. S.: Structural insights into the p97-Ufd1-Npl4 complex. Proc. Natl. Acad. Sci. USA, 104, 467-472 (2007).
6. Promnares, K.; Komenda, J.; Bumba, L.; Nebesarova, J.; Vacha, F., and Tichy, M.: Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits. J. Biol. Chem., 281, 32705-32713 (2006).
7. Collins, R. F.; Beis, K.; Clarke, B. R.; Ford, R. C.; Hulley, M.; Naismith, J. H.; and Whitfield, C.: Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules. J. Biol. Chem.,281, 2144-2150 (2006).
8. Wolfe, C. L.; Warrington, J. A.; Treadwell, L., and Norcum, M. T.: A three-dimensional working model of the multienzyme complex of aminoacyl-tRNA synthetases based on electron microscopic placements of tRNA and proteins. J. Biol. Chem., 280, 38870-38878 (2005).
9. Bumba, L.; Tichy, M.; Dobakova, M.; Komenda, J., and Vacha, F.: Localization of the PsbH subunit in photosystem II from the Synechocystis 6803 using the His-tagged NiNTA Nanogold labeling. J. Struct. Biol., 152, 28-35 (2005)
10. Collins, R. F.; Frye, S. A.; Balasingham, S.; Ford, R. C.; Tonjum, T., and Derrick, J. P.: Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ. J. Biol. Chem., 280, 18923-18930 (2005).
11. Chatterji, A.; Ochoa, W. F.; Ueno, T.; Lin T., and Johnson, J. E.: A virus-based nanoblock with tunable electrostatic properties. Nano Lett.,5, 597-602 (2005).
12. Buchel, C.; Morris, E.; Orlova, E., and Barber, J.: Localisation of the PsbH subunit in photosystem II: a new approach using labelling of His-tags with a Ni(2+)-NTA gold cluster and single particle analysis. J. Mol. Biol., 312, 371-379 (2001).
13. Hainfeld, J. F.; Liu, W.; Halsey, C. M. R.; Freimuth, P., and Powell, R. D.: Ni-NTA-Gold Clusters Target His-Tagged Proteins. J. Struct. Biol., 127, 185-198 (1999).
14. Hainfeld, J. F.; Liu, W.; Joshi, V., and Powell R. D.: Nickel-NTA-Nanogold Binds his-Tagged Proteins. Microsc. Microanal., 8, (Suppl. 2: Proceedings) (Proceedings of Microscopy and Microanalysis 2002); Voekl, E.; Piston, D.; Gauvin, R.; Lockley, A. J.; Bailey, G. W., and McKernan, S., Eds.; Cambridge University Press, New York, NY, 2002, p. 832CD.
GoldiBlot™ HIS Western Blot Kit:Ni-NTA-纳米金颗粒
应用:
1.蛋白杂交检测带his标签的重组蛋白(染色时间1小时)
2.识别细胞裂解液或提取物中的his标签蛋白
3.证实转染细胞中his标签蛋白的表达
优点:
更快更灵敏
低背景
永久信号
品名 | 产品描述 | 货号 | 规格 |
GoldiBlot™ HIS Western Blot Kit | 用于在蛋白杂交中检测his重组蛋白的 | 2090 | 15个杂交 |
References:
- Dubendorff, J.; Cruz, M.; Gonzalez, C.; Hainfeld, J.; Liu, W.: Rapid Detection of His-tagged Proteins on Western Blots Proc. 47th Ann. Mtg., Amer. Soc. Cell Biol., 47; Pres. # 1918., poster # B265 (2007).