Chromatography technique
色谱技术
His tidine-tagged protein purification
Binding Capacity/ml Chromatography Medium
结合力/ ml色谱介质
Approx. 40 mg histidine-tagged protein
Metal Ion Capacity
金属离子容量
pH stability, operational
PH稳定性operational
注：树脂可以在不明显改变功能的情况下操作的pH范围
pH stability, CIP3
PH稳定性 CIP3
注：Ni²⁺剥离介质
Pressure/Flow Specification
压力/流量规格
Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Chemical stability
化学稳定性
注：Ni²⁺剥离介质
Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea
absin特供GE health care分装
Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.
In process scale applications: This resin is used to purify histidine-tagged (his-tagged) and untagged recombinant proteins even at large scales.
In research scale applications: This resin binds 40 mg his-tag protein/mL resin and is suitable for use in batch mode, with gravity flow, or when scalability is needed.
Meets industrial demands with security of supply, robust performance, and regulatory support.
Available as bulk resin and as prepacked columns for use with manual methods or a chromatography system.
Excellent flow properties provide great performance even at high flow rates.
High binding capacity resin with excellent flow properties
Ni Sepharose 6 Fast Flow (FF) is composed of cross-linked 6% agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.