Recombinant Human IL-13

Name

Recombinant Human IL-13 (Recombinant Human Interleukin-13; rHuIL-13)；重组人白细胞介素13

Synonyms

IL-13; P600

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1ng/ml, corresponding to a specific activity of >1.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4 with 5% trehalose.

Endotoxin

Less than 1EU/μg of rHuIL-13 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 20mM HCl to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Human Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 5 and secreted by many cell types, especially T helper type 2 (Th2) cells. The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four α-helix configuration. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts antiinflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Human, mouse and rat IL-3 share low homology, but have cross species activity.

Amino Acid Sequence

GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGR FN

产品编号

产品名称

包装

KL-5178-2μg

Recombinant Human IL-13

2μg

KL-5178-10μg

Recombinant Human IL-13

10μg

KL-5178-100μg

Recombinant Human IL-13

100μg

KL-5178-1mg

Recombinant Human IL-13

1mg

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说明书

1份