Recombinant Human Epiregulin

Name

Recombinant Human Epiregulin (Recombinant Human Epiregulin; rHuEpiregulin)；重组人表皮素

Synonyms

EREG; epiregulin; ER; proepiregulin

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuEpiregulin as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Epiregulin encoded by the EREG gene in humans, is a member of the EGF family of growth factors. This family also includes epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. Epiregulin is expressed mainly in the placenta and peripheral blood leukocytes and in certain carcinomas of the bladder, lung, kidney and colon. It stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. Additionally, it inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0kDa mature secreted sequence.

Amino Acid Sequence

VAQVSITKCS SDMNGYCLHG QCIYLVDMSQ NYCRCEVGYT GVRCEHFFL

产品编号

产品名称

包装

KL-5570-5μg

Recombinant Human Epiregulin

5μg

KL-5570-25μg

Recombinant Human Epiregulin

25μg

KL-5570-100μg

Recombinant Human Epiregulin

100μg

KL--5570-1mg

Recombinant Human Epiregulin

1mg

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说明书

1份