Recombinant Human DES1-3 IGF-1

Name

Recombinant Human DES1-3 IGF-1 (Recombinant Human DES1-3 Insulin-like Growth Factor 1; rHuDES1-3 IGF-1)；重组人DES1-3胰岛素样生长因子1

Synonyms

IGF1; IGF-1; Somatomedin A; Somatomedin C; IBP1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; somatomedin-C; Somatomedin-C

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 50EU/mg of rHuDES1-3 IGF-1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. DES(1-3)IGF-1, is a truncated variant of human IGF-1 with the tripeptide Gly-Pro-Glu absent from the N-terminus. It has been isolated from bovine colostrum, human brain and porcine uterus. The DES(1-3)IGF-1 probably results from post-translational cleavage of IGF-1. It has about 10-fold more potent than IGF-I at stimulating hypertrophy and proliferation of cultured cells, a consequence of much reduced binding to IGF-binding proteins, in turn caused by the absence of the glutamate at position 3. Clinical opportunities for DES(1-3)IGF-1 have not yet been evaluated, but could apply in catabolic states as well as for the treatment of inflammatory bowel diseases.

Amino Acid Sequence

TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA

产品编号

产品名称

包装

KL-5507-20μg

Recombinant Human DES1-3 IGF-1

20μg

KL-5507-100μg

Recombinant Human DES1-3 IGF-1

100μg

KL-5507-1mg

Recombinant Human DES1-3 IGF-1

1mg

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说明书

1份