Recombinant Rat IL-13, 113a.a.

Name

Recombinant Rat IL-13, 113a.a. (Recombinant Rat Interleukin-13, 113a.a.; rRtIL-13, 113a.a.)；重组大鼠白细胞介素13，113a.a.

Synonyms

ALRHMGC116789; BHR1interleukin-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5ng/ml, corresponding to a specific activity of >2.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 5% trehalose.

Endotoxin

Less than 1EU/μg of rRtIL-13, 113a.a. as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-13 (IL-13) is expressed by the IL13 gene and secreted by many cell types, especially T helper type 2 (Th2) cells. The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four α-helix configuration. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Mature rat IL-13 shares 59%, 75%, and 60% amino acid sequence identity with human, mouse, and rhesus IL-13, respectively.

Amino Acid Sequence

TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSMVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH

产品编号

产品名称

包装

KL-6299-2μg

Recombinant Rat IL-13, 113a.a.

2μg

KL-6299-10μg

Recombinant Rat IL-13, 113a.a.

10μg

KL-6299-100μg

Recombinant Rat IL-13, 113a.a.

100μg

KL-6299-1mg

Recombinant Rat IL-13, 113a.a.

1mg

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说明书

1份