Recombinant Human SPARC

Name

Recombinant Human SPARC (Recombinant Human Secreted Protein Acidic and Rich in Cysteine; rHuSPARC)；重组人富含半胱氨酸酸性分泌蛋白

Synonyms

SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0μg/mL, corresponding to a specific activity of >333IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 0.1EU/μg of rHuSPARC as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Secreted protein acidic and rich in cysteine (SPARC), also named as osteonectin or BM-40, is an acronym for "secreted protein, acidic and rich in cysteine". It is encoded by the SPARC gene in humans. The protein is a glycoprotein of 40kDa, (303 amino acid residues) and consists of 17a.a. signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is the founding member of a family of secreted matricellular proteins with similar domain structure. It is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion.

Amino Acid Sequence

APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI

产品编号

产品名称

包装

KL-7169-10μg

Recombinant Human SPARC

10μg

KL-7169-50μg

Recombinant Human SPARC

50μg

KL-7169-100μg

Recombinant Human SPARC

100μg

KL-7169-1mg

Recombinant Human SPARC

1mg

-

说明书

1份