The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.
Advantages • High efficiency and rapid recombination cloning • Produces high titer adenoviral stocks • Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo • Produces replication-incompetent virus for enhanced biosafety of the system • Amenable for use in high-throughput applications
Key Features • Gateway® Technology for efficient and rapid cloning • CMV promoter for high-level constitutive expression of gene of interest • Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the
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