pEF4/V5-His A, B, and C are 5.7 kb vectors derived from pEF1/V5-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain thefollowing elements:
The control plasmid, pEF4/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.
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