RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640
Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With Without
●L-glutamine●HEPES
●Phenol Red
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Product use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco◎RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).
CGMP manufacturing and quality system RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-034). This facility is registered with the FDA as a medical device manufacturer and is cetified to the ISO 13485 standard.
关键字: RPMI 1640培养基;RPMI 1640;北京;
北京诺为生物技术有限公司( NovoBiotechnologyCo.. Ltd.)是一家集研发、 生产培训、市场营销于一体 ,服务于干细胞和免疫学研究,产品覆盖分子生物学、细胞分选、细胞分析、细胞培养、干细胞和兔疫细胞功能检测等 领域的高新技术服务企业。公司在北京、天津、山东设有办事处和研发生产中心。诺为生物秉承“质量第一,服务专业” 的经营理念,以专业的眼光审视和引进“新产品,新技术”服务科学研究;以“发展和壮大中国生命科学产业”为使命,为广大生命科学工作者创造一流的科研成果 提供强有力的服务支撑体系。
诺为生物以业绩为导向的人性化管理、科学的绩效考核与薪酬设计体系以及与企业共成长企业文化,使得我们逐步建立起骨干稳定、达成任务和目标能力强的研发团队和市场营销团队。整个团队敬业、乐业、专业、诚信、团结、奋进,具有强大的执行力、凝聚力、创新力。欢迎广大英年才俊加入我们,开启您的职业生涯, 共同成长,共创未来美好生活
Do what you love and enjoy your life!