![](/CAS/GIF/4286-23-1.gif)
4-Isopropenylphenol synthesis
- Product Name:4-Isopropenylphenol
- CAS Number:4286-23-1
- Molecular formula:C9H10O
- Molecular Weight:134.18
![4-Isopropylphenol](/CAS/GIF/99-89-8.gif)
99-89-8
184 suppliers
$13.00/10g
![4-Isopropenylphenol](/CAS/GIF/4286-23-1.gif)
4286-23-1
101 suppliers
$34.00/100mg
![4-(2-hydroxypropan-2-yl)phenol](/CAS/GIF/2948-47-2.gif)
2948-47-2
11 suppliers
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Yield:2948-47-2 56.8 %Chromat. ,4286-23-1 9.7 %Chromat.
Reaction Conditions:
Stage #1:4-Isopropylphenol with 4-methylphenyl phosphate synthase;ATP;magnesium chloride;manganese(ll) chloride in aq. buffer; pH=8 at 30; for 2 h;Enzymatic reaction;
Stage #2: with ferredoxin reductase;ferredoxin;P450 monooxygenase;NADPH in aq. buffer; pH=8 at 30; for 2 h;Enzymatic reaction;
Stage #3: with phosphohydrolase in aq. buffer; pH=8 at 30; for 2 h;Enzymatic reaction;
Steps:
Enzymatic Assays
General procedure: Unless specified otherwise, all enzymatic assays were carriedout in 100 μL of 50 mM Tris·HCl buffer (pH 8.0) at 30 °C for 2 h, and thefinal concentration of each enzyme was 10 μM. Methanol (3× volume) wasadded to quench reactions. Precipitated proteins were removed by centrifugationat 20,000 × g for 10 min and the supernatants were used as LC-MSsamples. For reactions catalyzed by CreHI, the reaction mixture contained1 mM substrate, 20 mM MgCl2, 2 mM MnCl2, 2 mM ATP, and purified CreHand CreI. For CreJEF activity toward phosphorylated compounds (2′-9′), theCreHI reactions were used to generate the phosphorylated substrates. Next,CreJ, CreE, and CreF together with 3 mM NADPH were added into the individualpost-CreHI reaction mixture.For one-pot chemomimetic alkylphenol oxidation reactions, the reactionmixtures contained CreHI, CreJEF, and the two optional enzymes CreG (with2 mM NAD+) and CreC (with 2 mM NADP+), 1 mM substrate 2-7, and necessarycofactors, including 20 mM MgCl2, 2 mM MnCl2, 2 mM ATP, and 3 mMNADPH. After incubation at 30 °C for 2 h, CreD was added into each of theone-pot mixtures, followed by another 2-h incubation at 30 °C.
References:
Du, Lei;Dong, Sheng;Zhang, Xingwang;Jiang, Chengying;Chen, Jingfei;Yao, Lishan;Wang, Xiao;Wan, Xiaobo;Liu, Xi;Wangi, Xinquan;Huang, Shaohua;Cui, Qiu;Feng, Yingang;Liu, Shuang-Jiang;Li, Shengying [Proceedings of the National Academy of Sciences of the United States of America,2017,vol. 114,# 26,p. E5129 - E5137]
![Bisphenol A](/CAS/GIF/80-05-7.gif)
80-05-7
654 suppliers
$13.00/100g
![4-Isopropenylphenol](/CAS/GIF/4286-23-1.gif)
4286-23-1
101 suppliers
$34.00/100mg
![Methyltriphenylphosphonium bromide](/CAS/GIF/1779-49-3.gif)
1779-49-3
588 suppliers
$5.00/10g
![4'-Hydroxyacetophenone](/CAS/GIF/99-93-4.gif)
99-93-4
831 suppliers
$5.00/25g
![4-Isopropenylphenol](/CAS/GIF/4286-23-1.gif)
4286-23-1
101 suppliers
$34.00/100mg
![1-Isopropenyl-4-methoxybenzene](/CAS/GIF/1712-69-2.gif)
1712-69-2
54 suppliers
$45.00/5mg
![4-Isopropenylphenol](/CAS/GIF/4286-23-1.gif)
4286-23-1
101 suppliers
$34.00/100mg
![Phenol](/CAS/20180808/GIF/108-95-2.gif)
108-95-2
721 suppliers
$14.00/25g
![4-Isopropenylphenol](/CAS/GIF/4286-23-1.gif)
4286-23-1
101 suppliers
$34.00/100mg