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Butanenitrile, 4-fluoro-3-hydroxy- synthesis
- Product Name:Butanenitrile, 4-fluoro-3-hydroxy-
- CAS Number:1537-89-9
- Molecular formula:C4H6FNO
- Molecular Weight:103.09
Yield:1537-89-9 84%
Reaction Conditions:
with cell-free extract containing HheB2 in TMEG buffer in aq. acetate buffer at 20; pH=7.5; for 3 h;Enzymatic reaction;regioselective reaction;
Steps:
Enzymatic preparation of fluoro alcohols 2a, 2e-2g
General procedure: Racemic 1a (100 mg,1.3 mmol, 100 mM final concentration) was dissolved in a 12 mL of Tris-SO4 buffer (0.5 M, pH 7.5) followed by the addition of sodium salt of azide, cyanide, nitrite or chloride (2.0-6.5 mmol) and 1 mL of cell-free extract containing HheB2 in TEMG buffer. The reaction was performed at room temperature. The reaction mixture was extracted with ethyl acetate. The combined organic extracts were dried over Na2SO4, filtered and the solvents evaporated.
References:
Majeri? Elenkov, Maja;?i?ak, Mirjana;Smolko, Ana;Kne?evi?, Anamarija [Tetrahedron Letters,2018,vol. 59,# 4,p. 406 - 408] Location in patent:supporting information