Butanenitrile, 4-fluoro-3-hydroxy- synthesis

Yield:1537-89-9 84%

Reaction Conditions:

with cell-free extract containing HheB₂ in TMEG buffer in aq. acetate buffer at 20; pH=7.5; for 3 h;Enzymatic reaction;regioselective reaction;

Steps:

Enzymatic preparation of fluoro alcohols 2a, 2e-2g

General procedure: Racemic 1a (100 mg,1.3 mmol, 100 mM final concentration) was dissolved in a 12 mL of Tris-SO₄ buffer (0.5 M, pH 7.5) followed by the addition of sodium salt of azide, cyanide, nitrite or chloride (2.0-6.5 mmol) and 1 mL of cell-free extract containing HheB2 in TEMG buffer. The reaction was performed at room temperature. The reaction mixture was extracted with ethyl acetate. The combined organic extracts were dried over Na₂SO₄, filtered and the solvents evaporated.

References:

Majeri? Elenkov, Maja;?i?ak, Mirjana;Smolko, Ana;Kne?evi?, Anamarija [Tetrahedron Letters,2018,vol. 59,# 4,p. 406 - 408] Location in patent:supporting information