Daidzein-D6 synthesis

Yield:291759-05-2 60%

Reaction Conditions:

Stage #1: daidzeinwith [D]-sodium hydroxide;platinum on activated charcoal;water-d2 at 130; for 8 h;Inert atmosphere;
Stage #2: with formic acid at 130; for 1 h;Inert atmosphere;

Steps:

29 Example 29

Adding 10 mg of daidzein to the reaction vessel,200μL of heavy water,1mg Pt/C and 130μLdeuteriumSodium oxide solution,Filled with nitrogen and reacted at 130 ° C for 8 hours.Then, 130 μL of formic acid was added and reacted at 130 ° C for 1 hour.The reaction solution was collected and freeze-dried to obtain a solid.Dissolve the solid in ethanol,The filtrate was collected by filtration and dried to obtain a deuterated daidzein solid.Mass spectrometry detectionsixDeuterated daidzein (the main peak of the six major peaks),The mass spectrum and nuclear magnetic spectrum before and after the reaction are shown in Fig. 9.The structure is as follows,The yield was 60%.

References:

CN109879847,2019,A Location in patent:Paragraph 0111-0113