(+)-DIHYDROCARVONE MIXTURE OF ISOMERS synthesis
- Product Name:(+)-DIHYDROCARVONE MIXTURE OF ISOMERS
- CAS Number:5524-05-0
- Molecular formula:C10H16O
- Molecular Weight:152.23
2244-16-8
242 suppliers
$6.00/5g
5524-05-0
102 suppliers
$47.03/25ML
Yield:5524-05-0 95.6%
Reaction Conditions:
Stage #1: (R)-Carvonewith sodium formate;NADH in aq. phosphate buffer; pH=7; for 2 h;
Stage #2: with recombinant Escherichia coli Loop 1,2a-FDH in aq. phosphate buffer at 25; for 5 h;Reagent/catalyst;
Steps:
4.5. Biotransformations of (R)-Carvone
Biotransformations of (R)-carvone were carried out on two dierent scales: 15 mL for theevaluation of technical replicability and 0.7 L for the preparative biotransformation in the stirred-tankbio reactors. All biotrans formations were performed with 300 mM (R)-carvone and 450 mM sodium formate in 300 mM sodium phosphate buer (pH 7.0). Amberlite XAD4 with a wet mass ratio of 3:1to (R)-carvone was used for in situ substrate feeding and product removal. (R)-carvone and adsorbentresin (harmonic mean size 0.49-0.69 mm, uniformity coecient 2) were incubated for at least 2 hin buer (50% (v/v) of the total reaction volume) prior to the biotransformations. Reactions wereinitiated by the addition of the whole-cell biocatalysts resuspended in 300 mM sodium phosphatebuer and the samples were incubated at 25 °C.
References:
M?hler, Christoph;Burger, Christian;Kratzl, Franziska;Weuster-Botz, Dirk;Castiglione, Kathrin [Molecules,2019,vol. 24,# 14,art. no. 2550]
2111-75-3
119 suppliers
$569.50/20mg
5524-05-0
102 suppliers
$47.03/25ML
4680-24-4
35 suppliers
$108.00/100mg
5524-05-0
102 suppliers
$47.03/25ML