DPN I
- CAS No.
- 81295-14-9
- Chemical Name:
- DPN I
- Synonyms
- DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease Dpn I;RESTRICTION ENDONUCLEASES DPN I;dpn i from diplococcus pneumoniae;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
- CBNumber:
- CB0256555
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MFCD00166565
- MOL File:
- Mol file
SAFETY
Risk and Safety Statements
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
|---|---|
| WGK Germany | 3 |
DPN I price More Price(4)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | DPNI-RO | Dpn I from Diplococcus pneumoniae | 81295-14-9 | 1000units | $357 | 2024-03-01 | Buy |
| American Custom Chemicals Corporation | ENZ0008473 | Dpn I 95.00% | 81295-14-9 | 5MG | $451.58 | 2021-12-16 | Buy |
| Biorbyt Ltd | orb93993 | Dpn I | 81295-14-9 | 200units | $74.8 | 2021-12-16 | Buy |
| Biorbyt Ltd | orb93993 | Dpn I | 81295-14-9 | 1000units | $302.6 | 2021-12-16 | Buy |
DPN I Chemical Properties,Uses,Production
Uses
In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.
General Description
Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.
DPN I Preparation Products And Raw materials
Raw materials
Preparation Products
DPN I Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Career Henan Chemica Co | +86-0371-86658258 15093356674; | laboratory@coreychem.com | China | 30255 | 58 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 821-50328103-801 18930552037 | 3bsc@sina.com | China | 15848 | 69 |
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
| Shanghai Kanglang Biotechnology Co., Ltd. | 021-61998208 18217752821 | sales@klbio.cn | China | 9617 | 58 |
| Wuhan Chemstan Biotechnology Co., Ltd. | 027-65317797 15926423062 | 422450190@qq.com | China | 10054 | 58 |
| Shanghai Yuchun Biotechnology Co., Ltd | 021-021-52273538 17321241593 | 1503472510@qq.com | China | 5255 | 58 |
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