ヒト遺伝子組換オリゴペプチド-1 化学特性,用途語,生産方法
定義
本品は、大腸菌 E.coli または酵母の一種 Pichia pastoris によって産生させた単鎖の遺伝子組換ヒトペプチドであり、53個のアミノ酸からなる。出発遺伝子は、上皮成長因子のコードを持ち、ヒト細胞から直接単離されたものである。上皮成長因子は、ジスルフィド結合と糖の両方またはいずれか一方を含む最大で1027個のアミノ酸からなり、固有のアミノ酸配列を持つ。
化粧品の成分用途
皮膚コンディショニング剤
説明
An ophthalmic formulation of epidermal growth factor (urogastrone) has been launched
for stimulation of healing of the corneal epithelium following various corneal diseases.
化学的特性
Endotoxin < 0.1 ng per μg (1EU/ug) of EGF
使用
Epidermal Growth Factor, human, animal component free has been used:
- as a supplement in in LHC-8 medium to culture liver cell lines
- in the fetal bovine serum (FBS)-Dulbecco′s modified essential medium (DMEM) /F12 medium for primary culture of human glioma cells
- as an additive in the conditional medium of normal fibroblasts (NFs) to study its effect on the migration and invasion of endometrial cancer (EC) cells
- as a component in tumorsphere medium
主な応用
Epidermal Growth Factor (EGF) is a human protein that is involved in the regulation of cell growth, differentiation and survival. Recombinant EGF is an antigen binding molecule that can be used as a cancer treatment. Recombinant EGF has been shown to stimulate cellular transformation by binding to the epidermal growth factor receptor. It also has been shown to inhibit the growth of breast cancer cells with the HER2 gene mutation. The molecular weight of rec EGF is approximately 25 kDa and is composed of 165 amino acids. Recombinant EGF can be used in immunoassays, as a model system for studying biological properties, or as an antibody production tool.
一般的な説明
Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (～6 kDa), which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids.
? Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion.
? EGF is a member of a growth factor family, which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF.
? EGF is homologous to a sequence contained in a 19 kDa protein of vaccinia virus,
? which appears to utilize the EGF receptor to gain entry into cell? EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.
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ヒト遺伝子組換オリゴペプチド-1 上流と下流の製品情報
原材料
準備製品