o-Phthalaldehyde

o-Phthalaldehyde 속성

o-Phthalaldehyde MSDS

1,2-Phthalic dicarboxaldehyde

o-Phthalaldehyde C화학적 특성, 용도, 생산

개요

황색 결정. 녹는점 56~57℃. 어느 것이든 물 및 대개의 유기 용매에 녹는다. 석유 에테르에 잘 안 녹는다. 수증기 증류할 수 있다. 산화크롬(Ⅵ)으로 산화하면 프탈산이 된다.

화학적 성질

o-Phthalaldehyde is a pale yellow crystalline solid.

o-Phthalaldehyde is mainly used as a high-level disinfectant (a low-temperature chemical method) for heat-sensitive medical and dental equipment such as endoscopes and thermometers; in recent years, it has gained popularity as a safe and better alternative to glutaraldehyde.
There are some researches show, pH7.5 contains the sterilizing agent of o-phthalaldehyde 0.5%, and its sterilizing power, sterilization speed, stability and toxicity all are better than glutaraldehyde, can kill mycobacterium in the 5min, the bacterium number reduces by 5 logarithmic value, and o-phthalaldehyde is very stable, tasteless in pH3～9 scopes, non-stimulated to human nose, eye mucosa, and need not activate before using, various materials are had good consistency, have tangible microbiocidal activity.

용도

o-Phthalaldehyde can be widely used for precolumn derivatization of amino acids in HPLC separation or Capillary electrophoresis. For flow cytometric measurements of protein thiol groups.

정의

ChEBI: A dialdehyde in which two formyl groups are attached to adjacent carbon centres on a benzene ring.

정의

Using o-phthalaldehyde (OPA) in combination with a thiol reagent is a standard method for detecting primary amines in amino acids, peptides, and proteins. O-phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers. Despite its widespread use, the exact reaction mechanism has been debated since the 1980s. Rovelli's results support the mechanism originally proposed by Sternson and Wong, in which the primary amine first reacts with OPA, followed by a reaction with the thiol to form the fluorescent isoindole product[1-2].

제조 방법

o-Phthalaldehyde is a high-level chemical disinfectant that is commonly used for disinfection of dental and medical instruments as an alternative to glutaraldehyde, which is a known skin and respiratory sensitizer.
A variety of processes for manufacturing o-phthalaldehyde have been reported in the literature.
o-Phthalaldehyde is produced by heating pure benzaldehyde and chloroform with potassium hydroxide solution. The resulting solution is further acidified with hydrochloric acid and cooled to yield a colorless powder of o-phthalaldehyde.
It is also produced by ozonization of naphthalene in alcohol followed by catalytic hydrogenation.
Catalytic oxidation of various chemicals is also used in manufacturing o-phthalaldehyde. o-Phthalaldehyde can be manufactured by oxidation of phthalan by nitrogen monoxide in acetonitrile with N-hydroxyphthalimide as the catalyst to yield 80% to 90%.

Biotechnological Applications

O-phthalaldehyde(OPA) is used for precolumn derivatization of amino acids for HPLC separation and for flow cytometric measurements of protein thiol groups. Used for fluorometric determination of histamine, histidine and other amino acids. Also used for cholesterol assay in the picomole range.
Phthaldialdehyde has been used:
in the preparation of O-phthaldialdehyde reagent for analysing gentamycin content.
in the preparation of reagent for determining the degree of hydrolysis of milk proteins.
in the measurement of free amino acids of milk samples by O-phthaldialdehyde/N-acetyl-L-cysteine (OPA/NAC) assay.
in the derivatization of putrescine samples.

잠재적 노출

The primary routes of human exposure to o-phthalaldehyde are by inhalation and through the skin, which may occur through accidental or occupational exposures. Along with its increasing popularity as a chemical sterilizer, o-phthalaldehyde has many applications in analytical methods and in diagnostic kits. o-Phthalaldehyde is also used as an intermediate in the synthesis of pharmaceuticals and as a reagent in the tanning industry, hair colorings, wood treatment, and antifouling paints. o-Phthalaldehyde was approved for use as an indoor antimicrobial pesticide in 1997; however, it is no longer registered with the United States Environmental Protection Agency (USEPA) for this use.

Carcinogenicity

No information on the carcinogenicity of o-phthalaldehyde in experimental animals or humans was found in a review of the literature.

o-Phthalaldehyde 준비 용품 및 원자재

원자재

준비 용품

o-Phthalaldehyde 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Jinan Finer Chemical Co., Ltd	+86-531-88989536 +86-15508631887	sales@finerchem.com	China	2966	58
Univar Solutions(China) Co., Ltd.	+8615902132654	ivy.zhuang@univarsolutions.com	China	654	58
Jinan Qinmu Fine Chemical Co.,Ltd.	+8618660799346	sales@qinmuchem.com	China	1009	58
Henan Tianfu Chemical Co.,Ltd.	+86-0371-55170693 +86-19937530512	info@tianfuchem.com	China	21689	55
Changzhou Zechong Biopharma Co., Ltd.	+undefined18621330623	sales@zechongchem.com	China	28	58
Mainchem Co., Ltd.	--	sarah@mainchem.com	China	6572	58
Henan Bao Enluo International TradeCo.，LTD	+86-17331933971 +86-17331933971	deasea125996@gmail.com	China	2503	58
Shaanxi Haibo Biotechnology Co., Ltd	+undefined18602966907	qinhe02@xaltbio.com	China	1000	58
Shandong Juchuang Chemical Co., LTD	+undefined15030412209	admin@juchuangchem.com	China	387	58
Shaanxi TNJONE Pharmaceutical Co., Ltd	+8618740459177	sarah@tnjone.com	China	1142	58

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