6-丙烯酰基-2-二甲氨基萘
中文名称 | 6-丙烯酰基-2-二甲氨基萘 |
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中文同义词 | 6-丙烯酰基-2-二甲氨基萘;1-(6-(二甲基氨基)萘-2-基)丙-2-烯-1-酮;6-丙烯酰-2-二甲氨基萘 |
英文名称 | 6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE |
英文同义词 | 2-Propen-1-one, 1-(6-(dimethylamino)-2-naphthalenyl)-;1-(6-(Dimethylamino)naphthalen-2-yl)prop-2-en-1-one;1-(6-Dimethylamino-naphthalen-2-yl)-propenone;ACRYLODAN;6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE;ACRYLODAN [6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE];1-(6-(Dimethylamino)-2-naphthalenyl)-2-propen-1-one;Acrylodan [6-Acryloyl-2-dimethylaminophthalene] |
CAS号 | 86636-92-2 |
分子式 | C15H15NO |
分子量 | 225.29 |
EINECS号 | |
相关类别 | |
Mol文件 | 86636-92-2.mol |
结构式 |
6-丙烯酰基-2-二甲氨基萘 性质
熔点 | 130-131 °C(Solv: ethanol (64-17-5); water (7732-18-5)) |
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沸点 | 388.3±24.0 °C(Predicted) |
密度 | 1.100±0.06 g/cm3(Predicted) |
储存条件 | 2-8°C |
酸度系数(pKa) | 4.32±0.40(Predicted) |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M
−1
cm
−1
for actin at 290 nm, 18,500 M
−1
cm
−1
or acrylodan at 385 nm, and 71,000 M
−1
cm
−1
for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 (
Acryl
Act1
NAT
) is dramatically different than that of EDTA-unfolded
Acryl
Act1 (
Acryl
Act1I3), which has a very similar emission spectrum to CCT-bound
Acryl
Act1.
安全信息
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
---|---|---|---|---|---|
2024/04/30 | HY-W040230 | Acrylodan | 10 mg | 1240元 | |
2024/01/25 | HY-W040230 | 6-丙烯酰基-2-二甲氨基萘 Acrylodan | 86636-92-2 | 5mg | 800元 |