| Identification | More | [Name]
Bromophenol Blue | [CAS]
115-39-9 | [Synonyms]
3',3'',5',5''-TETRABROMOPHENOLSULFONEPHTHALEIN
3',3'',5',5''-TETRABROMOPHENOLSULFONPHTHALEIN
3,3',5,5'-TETRABROMOPHENOLSULFONPHTHALEIN
3',3'',5'5''-TETRABROMOPHENOL-SULFONTHALEIN
3',3'',5',5''-TETRABROMOPHENOLSULFOPHTHALEIN
3,3-BIS(3,5-DIBROMO-4-HYDROXYPHENYL)-2,1LAMBDA6-BENZOXATHIOLE-1,1(3H)-DIONE
A,A-BIS(3,5-DIBROMO-4-HYDROXYPHENYL)-A-HYDROXY-O-TOLUENESULFONIC ACID G-SULTONE
ALBUTEST
ALPHA,ALPHA-BIS(3,5-DIBROMO-4-HYDROXYPHENOL)-ALPHA-HYDROXY-O-TOLUENESULFONIC ACID
BPB
BROMOPHENOL BLUE
BROMOPHENOL BLUE ETHANOL(50)
BROMOPHENOL BLUE LOADING SOLUTION
BROMOPHENOL BLUE SULFONE FORM
BROMOPHENOL BLUE TS
BROMPHENOL BLUE
BROMPHENOL BLUE SULTONE FORM
NSC 7818
PHENOL, 4,4'-(1,1-DIOXIDO-3H-2,1-BENZOXATHIOL-3-YLIDENE)BIS[2,6-DIBROMO-
PHENOL, 4,4'-(3H-2,1-BENZOXATHIOL-3-YLIDENE)BIS[2,6-DIBROMO-, S,S-DIOXIDE | [EINECS(EC#)]
204-086-2 | [Molecular Formula]
C19H10Br4O5S | [MDL Number]
MFCD00005875 | [Molecular Weight]
669.96 | [MOL File]
115-39-9.mol |
| Chemical Properties | Back Directory | [Appearance]
pink or red powder | [Melting point ]
273 °C
| [density ]
0.954 g/mL at 20 °C
| [Fp ]
58 °C | [storage temp. ]
Store at RT. | [solubility ]
methanol: soluble10mg/mL | [Boiling point ]
605.6±55.0 °C(Predicted) | [form ]
Solid | [pka]
3.85(at 25℃) | [color ]
Reddish-violet | [Odor]
Odorless | [PH]
pH : 3.0～4.6 | [PH Range]
3(Yellow)-4.6(Blue) 6(yellow)-7.6(blue) | [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
Freely soluble in NaOH. Soluble in sodium hydroxide, alcohol, benzene, and acetic acid. Slightly soluble in water. | [λmax]
598nm, 592nm, 436nm, 422nm, 273nm | [Merck ]
14,1444 | [BRN ]
61698 | [Major Application]
Nanotechnology, semiconductors, sol-gel matrix, fuel cells, display device, paints, packaging system, diapers, contact lens, determining nucleic acids, in saliva, water alkalinity, preserving genetic materials, detecting bacteria, vaginal infection test method | [InChIKey]
UDSAIICHUKSCKT-UHFFFAOYSA-N | [LogP]
6.880 (est) | [CAS DataBase Reference]
115-39-9(CAS DataBase Reference) | [EPA Substance Registry System]
Bromophenol blue (115-39-9) |
| Safety Data | Back Directory | [Hazard Codes ]
T,Xn | [Risk Statements ]
R61:May cause harm to the unborn child.
R20/21:Harmful by inhalation and in contact with skin .
R36:Irritating to the eyes.
R36/37/38:Irritating to eyes, respiratory system and skin .
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed . | [Safety Statements ]
S53:Avoid exposure-obtain special instruction before use .
S45:In case of accident or if you feel unwell, seek medical advice immediately (show label where possible) .
S24/25:Avoid contact with skin and eyes .
S22:Do not breathe dust .
S36:Wear suitable protective clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [RIDADR ]
UN 2265 3/PG 3
| [WGK Germany ]
3
| [RTECS ]
SJ7453000
| [TSCA ]
Yes | [HazardClass ]
3 | [PackingGroup ]
III | [HS Code ]
29349990 | [Hazardous Substances Data]
115-39-9(Hazardous Substances Data) |
| Hazard Information | Back Directory | [Chemical Properties]
Bromophenol Blue is pink or red powder
| [Uses]
As indicator, pH 3.0 yellow; pH 4.6 purple. | [Uses]
Bromophenol Blue is an electrophoresis tracking dye also used as an acid-base indicator
| [Definition]
Bromophenol Blue is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis. It has also been used as an industrial dye.
| [Preparation]
Synthesis of bromophenol blue: Dissolve phenol red in glacial acetic acid, add a solution of bromine in glacial acetic acid with stirring, pour into 60°C hot water after stirring for a few minutes, cool to room temperature, and place overnight. Filter, wash the filter cake with glacial acetic acid and benzene in turn, and air dry to obtain bromophenol blue. | [Biotechnological Applications]
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier. | [storage]
Room temperature | [Purification Methods]
Crystallise the dye from *C6H6 or Me2CO/AcOH, and dry it in air. It is an indicator: at pH 3.0 it is yellow and it is purple at pH 4.6. [Beilstein 19/3 V 458.] |
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