Identification | More | [Name]
Bromocresol Purple | [CAS]
115-40-2 | [Synonyms]
3',3''-DIBROMO-O-CRESOLSULFONPHTHALEIN 5',5''-DIBROMO-2-CRESOLSULFONEPHTHALEIN 5',5''-DIBROMO-O-CRESOLSULFONEPHTHALEIN 5',5'-DIBROMO-O-CRESOLSULFONEPHTHALEIN 5',5''-DIBROMO-O-CRESOLSULFONPHTHALEIN 5,5'-DIBROMO-O-CRESOLSULFONPHTHALEIN A,A-BIS(5-BROMO-4-HYDROXY-M-TOLYL)-A-HYDROXY-O-TOLUENESULFONIC ACID G-SULTONE BCP BROMCRESOL PURPLE BROMCRESOL PURPLE SULTONE FORM BROMOCRESOL PURPLE BROMOCRESOL PURPLE ETHANOL(20) BROMOCRESOL PURPLE TS DIBROMO-O-CRESOLSULFONPHTHALEIN 4,4’-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromo-6-methylphenol)s,s-dioxide 4,4’-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromo-6-o-cresol)s,s-dioxide 4,4’-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-bromo-b-methylphend]S,S-dioxide bromocresol Bromocresolred dibromo-o-cresolphthaleinsulfone | [EINECS(EC#)]
204-087-8 | [Molecular Formula]
C21H16Br2O5S | [MDL Number]
MFCD00011681 | [Molecular Weight]
540.22 | [MOL File]
115-40-2.mol |
Chemical Properties | Back Directory | [Appearance]
Crystalline | [Melting point ]
240 °C (dec.) (lit.) | [density ]
1.6509 (estimate) | [Fp ]
36 °C | [storage temp. ]
Store at RT. | [solubility ]
ethanol: soluble1mg/mL | [Boiling point ]
590.2±50.0 °C(Predicted) | [form ]
Solid | [pka]
6.21, 6.3, 6.4(at 25℃) | [color ]
Purplish-brown | [PH]
pH : 5.2~6.8 | [PH Range]
5.2(yellow)-6.8(purple) | [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
practically insoluble | [λmax]
419nm, 433nm, 591nm | [Merck ]
14,1387 | [BRN ]
359618 | [Major Application]
Waveguides, fuel cells, sensors, sol-gel technology, display device, falsification-proof security paper, paints, detergents, identifying fresh and stale rice, detecting lactic acid bacteria, dental materials, vaginal infection test method | [InChIKey]
ABIUHPWEYMSGSR-UHFFFAOYSA-N | [CAS DataBase Reference]
115-40-2(CAS DataBase Reference) | [EPA Substance Registry System]
115-40-2(EPA Substance) |
Safety Data | Back Directory | [Hazard Codes ]
Xi,Xn | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . R10:Flammable. R20/21/22:Harmful by inhalation, in contact with skin and if swallowed . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . S36:Wear suitable protective clothing . S37/39:Wear suitable gloves and eye/face protection . S16:Keep away from sources of ignition-No smoking . | [RIDADR ]
UN 1993 3/PG 3 | [WGK Germany ]
3
| [TSCA ]
Yes | [HS Code ]
29349990 | [Hazardous Substances Data]
115-40-2(Hazardous Substances Data) |
Hazard Information | Back Directory | [Chemical Properties]
Crystalline | [Uses]
As indicator, pH 5.2 yellow; pH 6.8 purple. | [Definition]
ChEBI: A member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-methylphenyl groups. A hydrophilic dye that is used as a pH indicator and to measure
serum albumin concentrations. | [General Description]
Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts. | [Biochem/physiol Actions]
Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts. | [Purification Methods]
Dissolve the dye in aqueous 5% NaHCO3 solution and precipitate it from a hot solution by dropwise addition of aqueous HCl. Repeat this until the UV/VIS-extinction did not increase at 419nm. It can also be recrystallised from *benzene. It is an indicator: at pH 5.2 (yellow) and pH 6.8 (purple). [Beilstein 19/3 V 460.] |
|
|