| Identification | Back Directory | [Name]
α-Glycerophosphate dehydrogenase | [CAS]
9075-65-4 | [Synonyms]
GDH
ALPHA-GDH
EC 1.1.1.8
dehydrogenase,glycerolphosphate
Α-GLYCEROPHOSPHATE DEHYDROGENASE
A-GLYCEROPHOSPHATE DEHYDROGENASE
GLYCEROL-3-PHOSPHATE DEHYDROGENASE
Glycerol-3-PhosphateDehydrogenaseEx
SN-GLYCERO-3-PHOSPHATE DEHYDROGENASE
ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE
ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE TYPE I
ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE TYPE X
GLYCEROL 3-PHOSPHATE DEHYDROGENASE extrapure
SN-GLYCEROL-3-PHOSPHATE: NAD 2-OXIDOREDUCTASE
SN-GLYCEROL-3-PHOSPHATE:NAD+ 2-OXIDOREDUCTASE
G-3-Pdh,AmmoniumSulfateSuspension,RabbitMuscle
Native Rabbit α-Glycerophosphate Dehydrogenase
Native Rabbit Glycerol-3-phosphate dehydrogenase
A-glycerophosphate dehydrogenase type I from rabb
sn-Glycero-3-phosphate Dehydrogenase rabbit muscle
A-GLYCEROPHOSPHATE DEHYDROGENASE TYPE X FROM RABBIT
A-GLYCEROPHOSPHATE DEHYDROGENASE TYPE I FROM RABBIT
α-glycerophosphate dehydrogenase from rabbit muscle
Glycerol 3-Phosphate Dehydrogenase,Rabbit muscle
α-Glycerophosphate Dehydrogenase From Rabbit Muscle
A-GLYCEROPHOSPHATE DEHYDROGENASE TYPE*II I FROM RABB
SN-GLYCEROL-3-PHOSPHATE:NAD+ 2-OXIDOREDUCTASE TYPE I
SN-GLYCEROL-3-PHOSPHATE:NAD+ 2-OXIDOREDUCTASE TYPE X
sn-glycero-3-phosphate dehydrogenase from rabbit muscle
sn-glycerol-3-phosphate dehydrogenase from rabbit muscle
Glycerol-3-phosphate dehydrogenase from rabbit, lyophil.
alpha-Glycerophosphate dehydrogenase (from Rabbit muscle)
glycerol-3-phosphate dehydrogenase from rabbit musc~270U/mg
Glycerol-3-Phosphate Dehydrogenase from E. coli, Recombinant
Glycerol-3-Phosphate Dehydrogenase ex. Rabbit Muscle, 15U/mg
GLYCERIN-3-PHOSPHAT-DEHYDROGENASE AUS KANI NCHENMUSK.120U/MG*
ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE, FROM RABBIT MUSCLE(ALPHA-GDH)
Native Rabbit α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase
alpha-GDH, Glycerol-3-phosphate dehydrogenase (NAD+), sn-Glycerol-3-phosphate: NAD+ 2-oxidoreductase
sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase, sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle
α-Glycerophosphate Dehydrogenase from rabbit muscle, GDH, sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase
α-Glycerophosphate Dehydrogenase from rabbit muscle,sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase | [EINECS(EC#)]
232-982-3 | [Molecular Formula]
NULL | [MDL Number]
MFCD00130487 |
| Hazard Information | Back Directory | [Uses]
α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:
- in the reaction mixture to measure the glycerol kinase activity
- to demonstrate compartmentalized enzymatic reactions where NADH is involved
- in the reaction mixture assay for L-fuculose-1-phosphate aldolase
| [Uses]
α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay. | [General Description]
α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase. | [Biochem/physiol Actions]
α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism. | [Purification Methods]
The dehydrogenase is recrystallised by adding (NH4)2SO4 to 0.45 saturation at pH 5.5 at 4oC, and the small amount of precipitate is removed, then a saturated solution of (NH4)2SO4 is added dropwise from time to time over several days in the cold room. The crystals are collected and recrystallised until they have maximum activity. The enzyme is stable in half-saturated (NH4)2SO4 for several weeks at 4o. The equilibrium [dihydroxyacetone][NADH][H+]/[G-3-P][NAD] is 1.0 x 10 -12M in Tris buffer at 25o. It uses NAD ten times more efficiently than NADP. The Km for G-3-P is 1.1 x 10 -4M, for NAD it is 3.8 x 10-4M and for dihydroxyacetone it is 4.6 x 10-4M in phosphate buffer pH 7.0 and at 23.3o. Dihydroxyacetone phosphate and fructose-1,6-diphosphate are inhibitors. [Branowski J Biol Chem 180 515 1949, The Enzymes 7 85 1963, Young & Pace Arch Biochem Biophys 75 125 1958, Walsh & Sallach Biochemistry 4 1076 1965.] |
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