| Identification | Back Directory | [Name]
LAMBDA PHAGE DNA | [CAS]
91080-14-7 | [Synonyms]
VIRAL DNA
LAMBDA DNA
DNA, LAMBDA
LAMBDA DAM- DNA
VIRAL LAMBDA-DNA
LAMBDA PHAGE DNA
DNA PHAGE LAMBDA
PHAGE LAMBDA DNA
DNA (LAMBDA PHAGE)
λ DNA, rm-, dam-, dcm-
LAMBDA DNA (DAM-, DCM-)
UNMETHYLATED LAMBDA DNA
LAMBDA PHAGE DNA, METHYLATED
DEOXYRIBONUCLEIC ACID, LAMBDA
LAMBDA PHAGE DNA, NON-METHYLATED
PHAGE LAMBDA DEOXYRIBONUCLEIC ACID
DEOXYRIBONUCLEIC AC. BACTERIOPH. (LAMB&
Deoxyribonucleic acids, bacteriophage lambda
DEOXYRIBONUCLEIC ACID, BACTERIOPHAGELAMB DA PHAGE
DEOXYRIBONUCLEIC ACID, BACTERIOPHAGELAMB DA PHAGE A
DEOXYRIBONUCLEIC ACID BACTERIOPHAGE*FROM LAMBDA PHA
DEOXYRIBONUCLEIC ACID METHYLATED, BACTERIOPHAGE (LAMBDA PHAGE)
lambda phage dna,methylated from escherichia coli host strain w3110
Deoxyribonucleic acid, bacteriophage (Lambda Phage) from Escherichia coli
Deoxyribonucleic acid methylated, bacteriophage (Lambda Phage) from E.coli
lambda phage dna,non-methylated from escherichia coli host strain gm119(rm-,dam-,dcm-)
Lambda Phage DNA, methylated solution from Escherichia coli host strain GM119 (rm-,dam-,dcm-)
Deoxyribonucleic acid methylated, bacteriophage (Lambda Phage) from E. coli host strain W3110 | [EINECS(EC#)]
293-505-2 | [MDL Number]
MFCD00146648 |
| Hazard Information | Back Directory | [Uses]
Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 is suitable for use as a substrate for restriction enzymes. It was used for immunoprecipitation in ChIP assays. | [Uses]
Lambda Phage DNA, Non-methylated from Escherichia coli host strain GM119 (rm-,dam-,dcm-) has been used:
- to calibrate spectrophotometer (LKB) and HPLC (high performance liquid chromatography) method for determination of bacterial genomic DNA G+C content
- to amplify the λ; exonuclease gene by polymerase chain reaction (PCR)
- in DNase activities
| [General Description]
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase. |
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Sigma-Aldrich
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021-61415566 800-8193336 |
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https://www.sigmaaldrich.cn |
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