单氯二胺
|
|
|
- CAS号:
- 76421-73-3
- 英文名:
- MONOCHLOROBIMANE
- 英文别名:
- MCB;MBCL;CHLOROBIMANE;THIOLYTE(R) MC;MONOCHLOROBIMANE;mBBr|||Chlorobimane;THIOLYTE(R) MC REAGENT;MBCl [MonochlorobiMane];Monochlorobimane Chlorobimane;THIOLYTE(R) MONOCHLOROBIMANE REAGENT
- 中文名:
- 单氯二胺
- 中文别名:
- 单氯二胺;单氯二胺 MBCL;MBCL [单氯二胺];3-(氯甲基)-2,5,6-三甲基吡唑并[1,2-A]吡唑-1,7-二酮
- CBNumber:
- CB3469396
- 分子式:
- C10H11ClN2O2
- 分子量:
- 226.66
- MOL File:
- 76421-73-3.mol
|
|
|
单氯二胺化学性质
-
熔点:
-
135-136 °C(lit.)
-
|
-
沸点:
-
314.9±44.0 °C(Predicted)
-
|
-
密度:
-
1.40±0.1 g/cm3(Predicted)
-
|
-
储存条件:
-
Keep in dark place,Inert atmosphere,2-8°C
-
|
-
溶解度:
-
DMF: soluble
-
|
-
酸度系数(pKa):
-
-3.22±0.70(Predicted)
-
|
-
形态:
-
Solid
-
|
-
颜色:
-
Light yellow to yellow
-
|
-
BRN:
-
4440901
-
|
单氯二胺性质、用途与生产工艺
Monochlorobimane (Chlorobimane) 是一种荧光染料 (λex=380 nm，λem=470 nm)，用于测定细胞中谷胱甘肽 (GSH)。
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of control and experimental wells:
The experiment should consist of parallel negative, positive and experimental wells respectively.
Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 µM H
2
O
2
)
2. Incubate the plate overnight at 37 °C, 5 % CO
2
. The incubation time varies depended on your normal protocol
3. Monochlorobimane (mBCl) is added to the cells at a final concentration of
20-100 μM
from a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20°C; the working solution was prepared before use by diluting the stock solution in 0.1 M PBS buffer (pH 7.0). In the assay the final concentration of DMSO was below 0.2% (v/v)
4. The cell suspensions were incubated with mBCl in the dark at 25°C for ~2 h
5. Remove the dye and treatment by centrifugation at 700 X g for 5 min. Add 200 µL of the Assay Buffer to each well and continue to the preferred method of detection
6. Detection of intracellular GSH:
Fluorescence plate reader: For adherent cells; read fluorescence directly off the culturing plate. If working with suspension cells; aliquot 200 µL of the culture suspension into the white opaque plate and record fluorescence at E
X
/E
M
= 380/465 ±20 nm respectively (
blue
).
单氯二胺
上下游产品信息
上游原料
下游产品
| 更新日期 | 产品编号 | 产品名称 | CAS编号 | 包装 | 价格 |
|---|
| 2024/08/19 | HY-101899 | Monochlorobimane | | 1 mg | 305元 |
| 2024/08/19 | HY-101899 | CHLOROBIMANE
Monochlorobimane | 76421-73-3 | 5mg | 610元 |
单氯二胺
生产厂家
全球有 45家供应商
单氯二胺国内生产厂家
76421-73-3, 单氯二胺 相关搜索:
- Other Fluorescent Labels
- Fluorescent Probes, Labels, Particles and Stains
- Fluorescent Labels
- Biochemicals and Reagents
- Antibody Labeling
- Labeling with FluorochromesFluorescent Probes, Labels, Particles and Stains
- DetectionAntibody Modification
- 抑制剂
- 实验室3
- 荧光染料
- 3-(氯甲基)-2,5,6-三甲基吡唑并[1,2-A]吡唑-1,7-二酮
- 单氯二胺 MBCL
- 单氯二胺
- MBCL [单氯二胺]
- 76421-73-3
- 3-(Chloromethyl)-2,5,6-trimethylpyrazolo[1,2-a]pyrazole-1,7-dione
- mBBr|||Chlorobimane
- 1H,7H-Pyrazolo[1,2-a]pyrazole-1,7-dione, 3-(chloromethyl)-2,5,6-trimethyl-
- Monochlorobimane, suitable for fluorescence, ≥70.0% (HPCE)
- Monochlorobimane Chlorobimane
- mBCl [Monochlorobimane] *CAS 76421-73-3*
- MBCl [MonochlorobiMane]
- MONOCHLOROBIMANE
- THIOLYTE(R) MC REAGENT
- THIOLYTE(R) MC
- THIOLYTE(R) MONOCHLOROBIMANE REAGENT, FLUORESCENT LABELING COMPOUND
- THIOLYTE(R) MONOCHLOROBIMANE REAGENT
- CHLOROBIMANE
- MCB
- MBCL