CHLOROBIMANE
中文名称 | CHLOROBIMANE |
---|---|
中文同义词 | MBCL [单氯二胺];单氯二胺;单氯二胺 MBCL |
英文名称 | MONOCHLOROBIMANE |
英文同义词 | MBCL;MCB;CHLOROBIMANE;THIOLYTE(R) MONOCHLOROBIMANE REAGENT;THIOLYTE(R) MONOCHLOROBIMANE REAGENT, FLUORESCENT LABELING COMPOUND;THIOLYTE(R) MC;THIOLYTE(R) MC REAGENT;MONOCHLOROBIMANE |
CAS号 | 76421-73-3 |
分子式 | C10H11ClN2O2 |
分子量 | 226.66 |
EINECS号 | |
相关类别 | 荧光染料;DetectionAntibody Modification;Labeling with FluorochromesFluorescent Probes, Labels, Particles and Stains;Antibody Labeling;Biochemicals and Reagents;Fluorescent Labels;Fluorescent Probes, Labels, Particles and Stains;Other Fluorescent Labels |
Mol文件 | 76421-73-3.mol |
结构式 |
CHLOROBIMANE 性质
熔点 | 135-136 °C(lit.) |
---|---|
沸点 | 314.9±44.0 °C(Predicted) |
密度 | 1.40±0.1 g/cm3(Predicted) |
储存条件 | Keep in dark place,Inert atmosphere,2-8°C |
溶解度 | 在DMF中可溶 |
酸度系数(pKa) | -3.22±0.70(Predicted) |
形态 | 固体 |
颜色 | 浅黄至黄色 |
BRN | 4440901 |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of control and experimental wells:
The experiment should consist of parallel negative, positive and experimental wells respectively.
Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 µM H
2
O
2
)
2. Incubate the plate overnight at 37 °C, 5 % CO
2
. The incubation time varies depended on your normal protocol
3. Monochlorobimane (mBCl) is added to the cells at a final concentration of
20-100 μM
from a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20°C; the working solution was prepared before use by diluting the stock solution in 0.1 M PBS buffer (pH 7.0). In the assay the final concentration of DMSO was below 0.2% (v/v)
4. The cell suspensions were incubated with mBCl in the dark at 25°C for ~2 h
5. Remove the dye and treatment by centrifugation at 700 X g for 5 min. Add 200 µL of the Assay Buffer to each well and continue to the preferred method of detection
6. Detection of intracellular GSH:
Fluorescence plate reader: For adherent cells; read fluorescence directly off the culturing plate. If working with suspension cells; aliquot 200 µL of the culture suspension into the white opaque plate and record fluorescence at E
X
/E
M
= 380/465 ±20 nm respectively (
blue
).
提供商 | 语言 |
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英文
|
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
---|---|---|---|---|---|
2024/04/30 | HY-101899 | Monochlorobimane | 1 mg | 305元 | |
2024/04/30 | HY-101899 | CHLOROBIMANE Monochlorobimane | 76421-73-3 | 5mg | 610元 |