化合物 T10325
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- CAS号:
- 931417-26-4
- 英文名:
- ANI-7
- 英文别名:
- ANI-7;ANI 7,ANI7;ANI-7, 10 mM in DMSO;(Z)-2-(3,4-Dichlorophenyl)-3-(1H-pyrrol-2-yl)acrylonitrile;(Z)-2-(3,4-Dichlorophenyl)-3-(1H-pyrrol-2-yl)acrylonitrile , ANI-7;Benzeneacetonitrile, 3,4-dichloro-α-(1H-pyrrol-2-ylmethylene)-, (αZ)-
- 中文名:
- 化合物 T10325
- 中文别名:
- 化合物ANI-7;化合物 T10325;化合物ANI-7,10 MM DMSO 溶液;(Z)-2-(3,4-二氯苯基)-3-(1H-吡咯-2-基)丙烯腈
- CBNumber:
- CB44667539
- 分子式:
- C13H8Cl2N2
- 分子量:
- 263.12
- MOL File:
- 931417-26-4.mol
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化合物 T10325化学性质
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沸点:
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443.0±45.0 °C(Predicted)
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密度:
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1.398±0.06 g/cm3(Predicted)
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储存条件:
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Store at -20°C
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溶解度:
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DMSO: 20.83 mg/mL (79.17 mM)
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酸度系数(pKa):
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15.79±0.50(Predicted)
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形态:
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Solid
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颜色:
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Light yellow to yellow
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化合物 T10325性质、用途与生产工艺
ANI-7 是一种 AhR 途径的激活剂。ANI-7 抑制多种癌细胞的生长，并有选择地抑制 MCF-7 乳腺癌细胞的生长，GI50 为 0.56 μM。ANI-7 通过激活 AhR 途径诱导 CYP1 代谢单加氧酶，并在敏感的乳腺癌细胞系中诱导 DNA 损伤，检查点激酶 (Chk2) 激活，S 期细胞周期停滞和细胞死亡。
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Aryl Hydrocarbon Receptor
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Chk2
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ANI-7 (2.5 μM; 24 hours; MCF10A and MDA-MB-468 cells) treatment induces significant S-phase and G2 + M-phase cell cycle arrest within 24 hours of treatment in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells.
ANI-7 (2 μM; 12-24 hours; MDA-MB-468 cells) treatment results in a significant increase in the content and phosphorylation of CHK2, and induces a significant increase in H2AXɣ in MDA-MB-468 cells, indicative of DNA double-strand damage.
Inhibition of the AhR pathway ameliorates the effects of ANI-7. ANI-7 activates XRE activity and expression of the AhR and CYP1 members.
Comparisons of the GI
50
values show that ANI-7 produces a GI
50
value of 0.38 μM in MCF-7 cells, whereas values of 3.0-42 μM are observed in cell lines from lung, colon, ovary, neuronal, glial, prostate, and pancreas. The only other tumor type that shows appreciable growth inhibition by ANI-7 is the A431 vulva cell line (GI
50
of 0.51μM).
ANI-7 potently inhibits the growth of T47D, ZR-75-1, MCF-7, SKBR3, and MDA-MB-468 breast cancer cells (GI
50
range of 0.16-0.38 μM), moderately inhibits the growth of BT20 and BT474 cells (GI50 range of 1-2 μM), and essentially fails to inhibit the growth of MDA-MB-231 and MCF10A cells (GI
50
range of 17-26 μM). Moreover, ANI-7 maintained its ability to inhibit the growth of drug-resistant cells (MCF-7/VP16: GI
50
of 0.21 μM).
Cell Cycle Analysis
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Cell Line:
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MCF10A and MDA-MB-468 cells
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Concentration:
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2.5 μM
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Incubation Time:
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24 hours
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Result:
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Induced significant S-phase and G2 + M-phase cell cycle arrest in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells.
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Western Blot Analysis
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Cell Line:
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MDA-MB-468 cells
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Concentration:
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2 μM
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Incubation Time:
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12 hours, 24 hours
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Result:
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Resulted in a significant increase in the content and phosphorylation of CHK2 (25-fold increase),and induced a significant increase in H2AXɣ (3.5-fold increase).
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化合物 T10325
上下游产品信息
上游原料
下游产品
| 更新日期 | 产品编号 | 产品名称 | CAS编号 | 包装 | 价格 |
|---|
| 2025/02/08 | HY-117102 | 化合物 T10325
ANI-7 | 931417-26-4 | 5mg | 700元 |
| 2025/02/08 | HY-117102 | 化合物 T10325
ANI-7 | 931417-26-4 | 10mM * 1mLin DMSO | 770元 |
931417-26-4, 化合物 T10325 相关搜索:
- 抑制剂
- 对照品
- 化合物ANI-7,10 MM DMSO 溶液
- (Z)-2-(3,4-二氯苯基)-3-(1H-吡咯-2-基)丙烯腈
- 化合物ANI-7
- 化合物 T10325
- 931417-26-4
- ANI-7, 10 mM in DMSO
- (Z)-2-(3,4-Dichlorophenyl)-3-(1H-pyrrol-2-yl)acrylonitrile
- (Z)-2-(3,4-Dichlorophenyl)-3-(1H-pyrrol-2-yl)acrylonitrile , ANI-7
- Benzeneacetonitrile, 3,4-dichloro-α-(1H-pyrrol-2-ylmethylene)-, (αZ)-
- ANI 7,ANI7
- ANI-7