Nuclease, Carbamidoylribo- Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Ribonuclease T1 (RNase T1) from
Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .
Biochem/physiol Actions
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.
Nuclease, Carbamidoylribo- Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
