AZ-27 Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
N-Cyclopropyl-5,6-dihydro-6-[4-[[[2-(2-oxa-7-azaspiro[3.5]non-7-yl)-3-pyridinyl]carbonyl]amino]benzoyl]-4H-thieno[3,2-d][1]benzazepine-2-carboxamide is a possible respiratory syncytial virus (RSV) RNA polymerase inhibitor.
Enzyminhibitor
This RSV inhibitor (FW = 633.77 g/mol) targets Respiratory Syncytial Virus, inhibiting both mRNA transcription and genome replication in cell based mini-genome assays, suggesting it targets a step common to both of these RNA synthesis processes. Analysis in an in vitro transcription run-on assay, containing RSV nucleocapsids, showed that AZ-27 inhibits (IC50 ≈ 15 nM) the synthesis of transcripts from the 3′-end of the genome to a greater extent than those from the 5′-end, indicating that it inhibits transcription initiation. In experiments assaying polymerase activity on the promoter, AZ-27 inhibits transcription and replication initiation. The RSV polymerase also utilizes the promoter sequence to perform a back-priming reaction. Addition of AZ-27 has no effect on the addition of up to three nucleotides by back-priming, but inhibits further extension of the back primed RNA. These findings suggest that the RSV polymerase is likely to adopt different conformations to execute its separate functions at the promoter.
AZ-27 Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
