ANTI-ACTIN Chemische Eigenschaften,Einsatz,Produktion Methoden
S-Sätze Betriebsanweisung:
S23:Gas/Rauch/Dampf/Aerosol nicht einatmen(geeignete Bezeichnung(en) vom Hersteller anzugeben).
S24/25:Berührung mit den Augen und der Haut vermeiden.
Verwenden
Rabbit anti-actin antibody can be used for western blot assays. The antibody can also be used for immunohistochemistry (1:25, using human or animal tissue sections) and indirect immunofluorescence (1:40, using chicken fibroblasts) applications.
Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates. Actin was detected at 42 kDa.
Allgemeine Beschreibung
Actin/ actin β(ACTB) is encoded by the gene mapped to human chromosome 7p15-p12. In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, αcardiac-actin, αsmooth-actin, and γ smooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
Biochem/physiol Actions
Actin is a cytoskeletal protein that regulates cell motility, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Mutations in the gene encoding this protein have been associated with Baraitser-Winter cerebrofrontofacial syndrome.
ANTI-ACTIN Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
