CARBOXYPEPTIDASE A
- CAS No.
- 11075-17-5
- Chemical Name:
- CARBOXYPEPTIDASE A
- Synonyms
- EC 3.4.17.1;E.C. 3.4.2.1;E.C. 3.4.12.2;CARBOXYPEPTIDASE A;CARBOXYPOLYPEPTIDASE;Carboxypeptidase A?Agarose;PEPTIDYL-L-AMINO-ACID HYDROLASE;CARBOXYPEPTIDASE A TYPE II-PMSF;CARBOXYPEPTIDASE A, PMSF TREATED;CarboxypeptidaseAExBovinePanceas
- CBNumber:
- CB1776427
- Molecular Formula:
- NULL
- Molecular Weight:
- 0
- MDL Number:
- MFCD00130718
- MOL File:
- Mol file
| Density | 1.00 g/mL at 20 °C |
|---|---|
| Flash point | 4 °C |
| storage temp. | 2-8°C |
| form | suspension |
| color | white |
| CAS DataBase Reference | 11075-17-5 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261-P284-P304+P340-P342+P311 |
| Hazard Codes | F,Xn,B,Xi |
| Risk Statements | 11-38-42-48/20-63-65-67-36-36/37/38 |
| Safety Statements | 36/37-45-62-36-26-23-16-24-22 |
| RIDADR | UN 1294 3/PG 2 |
| WGK Germany | 2 |
| F | 10 |
CARBOXYPEPTIDASE A price More Price(7)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | SAE0046 | Carboxypeptidase A from bovine pancreas (Type II-PMSF treated), ≥20?units/mg protein | 11075-17-5 | 500units | $46.8 | 2024-03-01 | Buy |
| Sigma-Aldrich | SAE0046 | Carboxypeptidase A from bovine pancreas (Type II-PMSF treated), ≥20?units/mg protein | 11075-17-5 | 2500Units | $133 | 2024-03-01 | Buy |
| Sigma-Aldrich | SAE0046 | Carboxypeptidase A from bovine pancreas (Type II-PMSF treated), ≥20?units/mg protein | 11075-17-5 | 5000units | $235 | 2024-03-01 | Buy |
| Sigma-Aldrich | C9268 | Carboxypeptidase A from bovine pancreas (Type II-PMSF treated), ≥50?units/mg protein, aqueous suspension | 11075-17-5 | 500units | $143 | 2024-03-01 | Buy |
| Sigma-Aldrich | C9268 | Carboxypeptidase A from bovine pancreas (Type II-PMSF treated), ≥50?units/mg protein, aqueous suspension | 11075-17-5 | 2500Units | $270 | 2024-03-01 | Buy |
CARBOXYPEPTIDASE A Chemical Properties,Uses,Production
Chemical Properties
Suspension (white)
Uses
The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.
Definition
An enzyme that catalyzes the cleavage of amino acids from a protein at the C-terminus of a polypeptide chain.
General Description
Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.
Industrial uses
Carboxypeptidase A(CPA) is an enzyme of the digestive system that is known to cleave amino acids favouring the C-terminal end as well as certain esters. This enzymatic activity depends on the metal at the catalytic site. Zn2+ and some Co2+-containing CPAs exhibit peptidase function, whilst esterase function has been seen by CPAs containing a variety of divalent d-block metals. CPA has a size similar to CA, consisting of about 300 amino acids and a molecular mass of 34 kDa.
Biochem/physiol Actions
Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.
Purification Methods
Carboxypeptidase A is purified by DEAE-cellulose chromatography, activation with trypsin and dialysed against 0.1M NaCl, yielding crystals. It is recrystallised by dissolving in 20 mL of M NaCl and dialysed for 24hours each against the following salts present in 500mL of 0.02M sodium veronal pH 8.0, 0.5M NaCl, 0.2M NaCl and 0.15M NaCl. The last dialysate usually induces crystallisation. If it does not crystallise, then dialyse the last solution against 0.02M sodium veronal containing 0.10M NaCl. Only 2 or 3 recrystallisations are required to attain maximum activity. [Cox et al. Biochemistry 3 44 1964.] Enzyme activity is measured by hydrolysing hippuryl-L-phenylalanine (or phenylacetic acid) and observing the rate of change of optical density at 254nm (reaction extinction coefficient is ~0.592 cm2/Wmole at pH 7.5) [Bergmyer Methods in Enzymatic Analysis (Academic Press) 1 436 1974].
CARBOXYPEPTIDASE A Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21634 | 55 |
| Hubei Jusheng Technology Co.,Ltd. | 18871490254 | linda@hubeijusheng.com | CHINA | 28172 | 58 |
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| Career Henan Chemica Co | +86-0371-86658258 +8613203830695 | laboratory@coreychem.com | China | 30240 | 58 |
| Shanghai Acmec Biochemical Technology Co., Ltd. | +undefined18621343501 | product@acmec-e.com | China | 33338 | 58 |
| Aladdin Scientific | +1-+1(833)-552-7181 | sales@aladdinsci.com | United States | 57505 | 58 |
| Wuhan Fortuna Chemical Co.,Ltd | +8618007136271 | hk@fortunachem.com | China | 5998 | 58 |
| SHANGHAI KEAN TECHNOLOGY CO., LTD. | +8613817748580 | cooperation@kean-chem.com | China | 40066 | 58 |
| Mainchem Co., Ltd. | -- | sarah@mainchem.com | China | 6567 | 58 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 821-50328103-801 18930552037 | 3bsc@sina.com | China | 15839 | 69 |
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