Biocytin
- CAS No.
- 576-19-2
- Chemical Name:
- Biocytin
- Synonyms
- BCT;BIOCYTIN;L-BIOCYTIN;d-Biocytin;H-LYS(BIOT)-OH;H-Lys(biotinyl);H-LYS(BIOTINYL)-OH;BIOCYTIN FREE BASE;Biocytin (εL-Lys(biotinyl)-OH
- CBNumber:
- CB5184561
- Molecular Formula:
- C16H28N4O4S
- Molecular Weight:
- 372.48
- MDL Number:
- MFCD00077319
- MOL File:
- 576-19-2.mol
- MSDS File:
- SDS
Melting point | ~245 °C (dec.) (lit.) |
---|---|
alpha | D25 +53° (c = 1.05 in 0.1N NaOH) |
Boiling point | 748.0±60.0 °C(Predicted) |
Density | 1.234 |
storage temp. | -20°C |
solubility | Aqueous Base (Slightly), Water (Slightly, Sonicated) |
form | Solid |
pka | 2.53±0.24(Predicted) |
color | White to Off-White |
optical activity | [α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.) |
Water Solubility | Soluble in water (50 mM) |
Merck | 13,1227 |
BRN | 97197 |
InChIKey | BAQMYDQNMFBZNA-MNXVOIDGSA-N |
FDA UNII | G6D6147J22 |
Biocytin price More Price(33)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich | B4261 | Biocytin ≥98% (TLC) | 576-19-2 | 5mg | $56.9 | 2024-03-01 | Buy |
Sigma-Aldrich | B4261 | Biocytin ≥98% (TLC) | 576-19-2 | 25mg | $104 | 2024-03-01 | Buy |
Cayman Chemical | 16751 | Biocytin ≥98% | 576-19-2 | 10mg | $37 | 2024-03-01 | Buy |
Cayman Chemical | 16751 | Biocytin ≥98% | 576-19-2 | 25mg | $72 | 2024-03-01 | Buy |
Sigma-Aldrich | B4261 | Biocytin ≥98% (TLC) | 576-19-2 | 50mg | $154 | 2024-03-01 | Buy |
Biocytin Chemical Properties,Uses,Production
Description
Biocytin is a conjugate of biotin and lysine known formally as ε-N-(d-biotinyl)-L-lysine. The addition of lysine to biotin increases the chain length extending from biotin, allowing the synthesis of medium-chain reagents. It also provides terminal carboxyl and amino groups for derivatization or conjugation to proteins and other molecules. Covalent linking reactions typically involve carbodiimide or NHS-ester crosslinking chemistries. Biocytin is also used as an anterograde, retrograde, or intracellular neuroanatomical tracer that is fixable with aldehyde-based fixatives. For this and other applications, biocytin is detected with avidin or streptavidin probes.
Chemical Properties
White Solid
Uses
Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.
Uses
Biocytin can be used as a versatile fluorescent marker for neuroanatomical investigations and for a biotinidase assay.
Uses
Useful for the synthesis of Biocytin containing peptides to study the mechanism of reactions catalyzed by biotin - containing enzymes
Definition
ChEBI: A monocarboxylic acid amide that results from the formal condensation of the carboxylic acid group of biotin with the N6-amino group of L-lysine.
Biological Activity
Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.
Biochem/physiol Actions
Biocytin is an adduct of lysine and biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme.
storage
-20°C
Purification Methods
Recrystallise biocytin rapidly from dilute MeOH or Me2CO. It can also be recrystallised from H2O by slow evaporation or by dissolving in the minimum volume of H2O and adding Me2CO until solid separates. It is freely soluble in H2O and AcOH but insoluble in Me2CO. [Wolf et al. J Am Chem Soc 74 2002 1952, 72 1048 1950.] It has been purified by chromatography on superfiltrol-Celite, Al2O3 and by countercurrent distribution and then recrystallised [IR: Peck et al. J Am Chem Soc 74 1991 1952]. The hydrochloride recrystallises from aqueous Me2CO/HCl and has m 227o (dec). [Beilstein 27 III/IV 7984.]
Biocytin Preparation Products And Raw materials
Raw materials
Preparation Products
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
ATK CHEMICAL COMPANY LIMITED | +undefined-21-51877795 | ivan@atkchemical.com | China | 32836 | 60 |
GenicBio Limited | +86-21-37621270 | service@genicbio.com | CHINA | 991 | 58 |
Hubei xin bonus chemical co. LTD | 86-13657291602 | linda@hubeijusheng.com | CHINA | 22968 | 58 |
CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49392 | 58 |
career henan chemical co | +86-0371-86658258 +8613203830695 | factory@coreychem.com | China | 29821 | 58 |
Shaanxi Dideu Medichem Co. Ltd | +86-29-87569266 15319487004 | 1015@dideu.com | China | 4088 | 58 |
Guangzhou Yuheng Pharmaceutical Technology Co., Ltd | +8613580539051 | joe@yuhengpharm.com | CHINA | 21149 | 58 |
Career Henan Chemica Co | +86-0371-86658258 +8613203830695 | laboratory@coreychem.com | China | 30250 | 58 |
GL Biochem (Shanghai) Ltd. | +86-021-61263370 +86-18901917034 | CHINA | 3904 | 58 | |
GL Biochem(Binhai) Ltd. | +86-021-61263370 +8615301650015 | wuqiuyan@glschina.com | China | 2975 | 58 |
View Lastest Price from Biocytin manufacturers
Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
---|---|---|---|---|---|---|---|---|
2024-04-02 | Biocytin
576-19-2
|
US $0.00 / g | 1g | >95.00% | 1g | Xian confluore Biological Technology Co., Ltd. | ||
2022-02-19 | H-Lys(biotinyl)-OH
576-19-2
|
US $0.00 / KG | 1KG | 98.9% | 100 tons | Honest Joy Holdings Limited
|
||
2020-01-09 | Biocytin
576-19-2
|
US $1.00 / g | 1g | ≥98% | g/kg/Ton | Career Henan Chemical Co |
- Biocytin
576-19-2
- US $0.00 / g
- >95.00%
- Xian confluore Biological Technology Co., Ltd.
- H-Lys(biotinyl)-OH
576-19-2
- US $0.00 / KG
- 98.9%
- Honest Joy Holdings Limited
- Biocytin
576-19-2
- US $1.00 / g
- ≥98%
- Career Henan Chemical Co
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