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DIG-HIGH PRIME

DIG-HIGH PRIME Structure
DIG-HIGH PRIME structure
CAS No.
Chemical Name:
DIG-HIGH PRIME
Synonyms
DIG-HIGH PRIME
CBNumber:
CB8684688
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD02098174
MOL File:
Mol file
Last updated:2023-04-23 13:52:06
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DIG-HIGH PRIME Properties

storage temp. -20°C
form solution

DIG-HIGH PRIME price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 11585606910 DIG-High Prime sufficient for 40?labeling reactions, pkg of 160?μL, solution 160μL $781 2024-03-01 Buy
American Custom Chemicals Corporation BOM0000135 DIG-HIGH PRIME 95.00% 5MG $498.98 2021-12-16 Buy
Product number Packaging Price Buy
11585606910 160μL $781 Buy
BOM0000135 5MG $498.98 Buy

DIG-HIGH PRIME Chemical Properties,Uses,Production

Uses

DIG-High Prime-labeled DNA probes has been used in a variety of hybridization techniques :

  • in Southern blots
  • in northern blots
  • in dot/slot blots
  • for screening of gene libraries
  • in In situ hybridizations
Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1μg total human DNA. The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots.

General Description

DIG-High Prime has enzyme and nucleotide mixture for rapid random-primed labeling of DNA with Digoxigenin-11-dUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. DIG-labeled probes are generated at high yield within one hour or after overnight incubation.

DIG-HIGH PRIME Preparation Products And Raw materials

Raw materials

Preparation Products

DIG-HIGH PRIME Suppliers

Global( 2)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
DIG-HIGH PRIME